IGF-1和Pit-1基因多态性与淮猪新品系生长性状的相关性分析  

IGF-1 and Pit-1 Genes Polymorphism and Analysis of Correlation of Growth Traits in New Strain Huai Pig

作　　者：许金根[1] 李庆岗[2] 刘林清[2] 陶立[2] 

机构地区：[1]安徽农业大学动物科技学院,安徽合肥230036 [2]安徽省农业科学院畜牧兽医研究所,安徽合肥230031

出　　处：《畜牧与饲料科学》2009年第9期100-103,共4页Animal Husbandry and Feed Science

基　　金：国家"863"计划项目(2007AA10Z171);国家支撑计划(2006BAD01A08-09和2008BADB2B02-11)

摘　　要：以淮猪新品系Ⅱ系一世代（190头）、二世代（244头）为试验材料,采用PCR-RFLP方法分别检测IGF-1基因的HhaⅠ酶切位点、Pit-1基因的RsaⅠ酶切位点的多态性,并与生长性状进行相关性分析。结果表明：30~90kg平均日增重、达90kg校正日龄在一世代、二世代、两个世代合并中,IGF-1基因的AA型个体均有优于AB、BB型个体的趋势,但差异都不显著（P＆gt;0.05）;Pit-1基因的DD型个体均有优于CD、CC型个体的趋势,但差异都不显著（P＆gt;0.05）。虽然IGF-1基因、Pit-1基因的不同基因型个体在一世代、二世代的达90kg校正背膘厚的趋势不一致,但是在两个世代合并中以IGF-1基因的AA型个体、Pit-1基因的DD型个体最薄,但差异不显著（P＆gt;0.05）。若将IGF-1基因、Pit-1基因作为生长性状的遗传标记用于淮猪新品系的选育,需要在淮猪新品系Ⅱ系后续世代中扩大样本含量并进行进一步的验证。he first-generation （190） and the second-generation （244） were selected as experimental materials from Ⅱ type of new strain Huai pig,and PCR-RFLP methods were used for detecting gene polymorphisms with Hha Ⅰ restriction enzyme sites of IGF-1 gene and Rsa Ⅰ restriction enzyme site of Pit-1,then the correlation between gene polymorphisms and partial performances were analyzed. The results showed that the average daily gain is 3090 kg,with adjust of days old,they attained 90 kg in the first and second generation. By combining two generations,AA genotype of IGF-1 gene had better tendency than AB and BB genotype,but the differences were not significant （P〉0.05） ,meanwhile DD genotype of Pit-1 gene had better tendency than CD and CC genotype,but the differences were not significant （P〉0.05）. After adjusted,their backfat thickness reached 90 kg,but tendency wasn′t inconsistent between the first and second generation. AA genotype of IGF-1 gene,DD genotype of Pit-1 gene was the thinnest combining two generations pigs,the differences were not significant （P〉0.05）. Whether IGF-1 gene and Pit-1 gene could be taken as genetic marker of growth traits to breed new strain Huai pig ,it still needs to be further validated in next generations expanding sample size in Ⅱ type of new strain Huai pig.

关 键 词：淮猪新品系 IGF-1基因 PIT-1基因 PCR-RFLP 多态性 

分 类 号：S828.2[农业科学—畜牧学]