氯化两面针碱在体外对肝细胞L-O2和肾细胞293增殖的影响  

In vitro nephrotoxicity of nitidine chloride in human liver L-O2 and kidney 293 cells

作　　者：韦敏[1] 刘华钢[2] 刘丽敏[2] 李丹妮[2] 

机构地区：[1]广西医科大学附属第五医院,柳州545005 [2]广西医科大学药学院,南宁530021

出　　处：《中国新药杂志》2010年第1期56-59,63,共5页Chinese Journal of New Drugs

基　　金：国家自然科学基金(30850010);广西科学研究与技术开发计划项目(桂科改0630002-2E)

摘　　要：目的:观察氯化两面针碱在体外对人胃癌细胞7901、人鼻咽癌细胞cne1-1、人肾癌细胞ketr-3、人肝癌细胞BEL 7404的增殖抑制作用和对人胚肝细胞L-O2和人胚肾细胞293的增殖影响。方法:采用MTT法检测不同浓度氯化两面针碱对4种肿瘤细胞和人胚肝细胞L-O2及人胚肾细胞293增殖的影响,比较其IC50值;采用紫外分光光度法测定氯化两面针碱作用后肝、肾细胞培养上清液中SOD,MDA,LDH值的变化。结果:氯化两面针碱在体外对肿瘤细胞具有较强的增殖抑制作用,同时对人胚肝细胞L-O2和人胚肾细胞293有一定的增殖抑制作用。结论:氯化两面针碱能抑制多种肿瘤细胞的增殖,对肿瘤细胞有明显细胞毒性作用,同时可抑制人胚肝细胞L-O2和人胚肾细胞293的增殖,对人体肝、肾细胞有一定的毒性。Objective：To study the in vitro antiproliferative effect of nitidine chloride on the human liver cell line L-O2 ceils and the human embryonic kidney cell line 293 cells. Methods： MTT assay was used to assess the antiproliferative effect of nitidine chloride in tumor cells, L-O2 cells and 293 cells. We compared their IC50 val- ues for the effect of nitidine chloride. The levels of SOD, MDA and LDH in cultural media were measured by ultraviolet spectrophotometry. Results： Nitidine chloride inhibited growth of tumor cells as well as L-O2 cells and 293 cells in a concentration-dependent manner. Conclusion： Nitidine chloride is cytotoxic in tumor cells, and inhibits the proliferation of L-O2 cells and 293 cells.

关 键 词：氯化两面针碱 入胚肝细胞L-O2 人胚肾细胞293 体外 MTT法 

分 类 号：R286.91[医药卫生—中药学]