抗凝护心Ⅱ号对急性心肌缺血再灌注大鼠P选择素的影响  

Effects of KNHX-Ⅱ on P-selectin after Acute Myocardial Ischemia and Reperfusion in Rats

作　　者：曹君娴[1] 陈波[2] 王秀娟[3] 

机构地区：[1]哈尔滨医科大学附属第一临床医院心内三科,黑龙江哈尔滨150001 [2]黑龙江中医药大学附属第一临床医院循环二科,黑龙江哈尔滨150020 [3]扬中市人民医院心内科,江苏扬中212200

出　　处：《中华中医药学刊》2010年第1期179-181,共3页Chinese Archives of Traditional Chinese Medicine

基　　金：哈尔滨医科大学附属第一临床医院科研基金资助项目(2007054)

摘　　要：目的:探求急性心肌缺血再灌注后,抗凝护心Ⅱ号减轻炎症反应的机理和疗效。方法:48只wistar大鼠随机分为6组,建立急性心肌梗死缺血再灌注模型。一组为假手术组,其余分别给予生理盐水、抗凝护心Ⅱ号低剂量、中剂量、高剂量和噻氯吡啶,取大鼠心肌组织行HE染色观察炎性细胞浸润程度,并用酶联免疫吸附试验检测大鼠血浆P选择素水平。结果:抗凝护心Ⅱ号低剂量组、中剂量组和高剂量组均可明显降低血浆P选择素水平(P<0.01),且给药浓度增加该作用增强。组织学观察表明,抗凝护心Ⅱ号可减轻再灌注后心肌组织炎性细胞浸润。结论:抗凝护心Ⅱ号可减轻由心肌缺血再灌注引起的炎症反应及相应的病理改变。Objective ： To study the mechanism and curative effectiveness of the KangNingHuXin （ KNHX - Ⅱ ） on reducing inflammatory reaction in myocardial ischemia and reperfusion. Methods： 48 rats were randomly allocated into 6 groups, which were established acute myocardial infarction and reperfusion models. One group was sham - operation group, others were given as follows ： sodium Chloride, low - dosage of KNHX - Ⅱ , middle - dosage of KNHX - Ⅱ , high - dosage of KNHX - Ⅱ and Panaldine group. P - selectin in the plasma of rats was measured. Results ： P - selectin in the plasma of rats greatly decreased in low - dosage of KNHX - Ⅱ ,middle - dosage of KNHX - Ⅱ and high - dosage of KNHX- Ⅱ compared with sham operation group （P 〈 0.01 ）. And with the dosage increasing, the effect was obvious. Histological observation showed that KNHX- Ⅱ could reduce the infiltration of inflammatory cells in myocardium after reperfusion. Conclusion： KNHX- Ⅱ could reduce inflammatory reaction and relevant pathological changes.

关 键 词：抗凝护心Ⅱ号 缺血再灌注损伤 P选择素 炎症 

分 类 号：R285.5[医药卫生—中药学]