左归丸对D-半乳糖致亚急性衰老大鼠ATP酶及Bcl-2 Bax和Caspase-3表达的影响  

The Effect of Zuogui Pill on Atpase Bcl-2 Bax and Caspase-3 of Subacute Aging Rats Induced by D-Galactose

作　　者：孙琳林[1] 康广盛[2] 马艳春[2] 

机构地区：[1]牡丹江医学院附属红旗医院中西医结合科,黑龙江牡丹江157011 [2]黑龙江中医药大学,黑龙江哈尔滨150040

出　　处：《中华中医药学刊》2010年第1期205-207,共3页Chinese Archives of Traditional Chinese Medicine

摘　　要：左归丸出自《景岳全书》,是公认的抗衰老古方,本研究旨在通过观察左归丸对D-半乳糖致亚急性衰老大鼠ATP酶及细胞凋亡相关因子Bcl-2、Bax和Caspase-3表达的影响,从实验的角度探讨抗衰老古方左归丸的延缓衰老作用机制。结果显示D-gal可以造成大鼠肝、脑组织中ATP酶活力降低、脑海马区的Bcl-2、Bax和Caspase-3表达升高,给予左归丸后,大鼠Na+-K+-ATPase和Ca2+-Mg2+-ATPase活力表现出不同程度的升高,Bcl-2的表达上调而Bax和Caspase-3表达下调,提示左归丸的抗衰老作用可能与其提高ATP酶活力以维持细胞膜的稳定性和抑制细胞凋亡作用有关。To observe the effect of Zuogui Pill on ATPase and expressions of Bcl-2, Bax and Caspase-3 which are agents related with apoptosis in subacute aging rats induced by D-galactose （D-gal）, to use Liuweidihuang Pill as control and to approach the function＇s mechanisms preliminarily of Zuogui Pill as an archaic anti-aging formula. D-gal can result in the cutting down of ATPase in the liver and brain of rats and the stepping up of the expression of Bcl-2, Bax and Caspase-3 in the hippocamp zone of rats＇brain. After the intervention of Zuogui Pill, the activity of Na＋ - K＋ - ATPase and Ca2 ＋ - Mg2＋ - ATPase and the expression of Bcl-2 rise in different degree and the expressions of Bax and Caspase-3 descend. The anti-aglng function of Zuogui Pill may be concerned with that this formula can raise the activity of ATPase to retain the stability of cell envelope and restrain apoptosis.

关 键 词：左归丸 衰老 ATP酶 细胞凋亡 

分 类 号：R285.5[医药卫生—中药学]