水通道蛋白3、9在特发性羊水过多产妇胎盘和胎膜中的表达变化及意义  

Expression of aquaporin 3 and aquaporin 9 in placenta and fetal membrane with idiopathic polyhydramnios

作　　者：朱雪琼[1] 蒋珊珊[1] 邹双微[1] 胡迎春[1] 王玉环[1] 

机构地区：[1]温州医学院附属第二医院妇产科,325027

出　　处：《中华妇产科杂志》2009年第12期920-923,共4页Chinese Journal of Obstetrics and Gynecology

基　　金：温州市对外合作交流科技计划（H20070042）

摘　　要：目的探讨水通道蛋白（AQP）3、9在特发性羊水过多产妇胎盘和胎膜中的表达、分布及其在特发性羊水过多发病中的作用。方法2006年6月至2008年3月，选择在温州医学院附属第二医院产科住院并分娩的21例特发性羊水过多的足月产妇为研究组，同期分娩的30例羊水量正常的足月产妇为对照组。采用实时荧光定量PCR技术，检测两组产妇胎盘、胎膜中AQP3、9mRNA的表达及分布，采用免疫绀化链霉菌抗生物素蛋白-过氧化物酶（SP）连接法榆测两组产妇胎氍、胎膜中AQP3、9蛋白的表达及分布。结果（1）两组产妇羊膜、绒毛膜、胎盘中均可检测到AQm、9mRNA的表达。AQP3、9主要表达于羊膜上皮细胞、绒毛膜滋养细胞、胎盘滋养细胞。（2）研究组羊膜七皮细胞AQP3、9mRNA的表达分别为对照组的5．00倍和3．25倍，而研究组绒毛膜滋养细胞AQP3、9mRNA的表达分别为对照组的2．03倍和2．08倍，分别比较，差异均有统计学意义（P〈0．01）；但是研究组胎盘滋养细胞AQP3、9mRNA的表达均明湿低于对照组，差异也有统计学意义（P〈0．01）。（3）AQV3、9蛋自在研究组羊膜上皮细胞的表达强度为7．5±2．0、11．1±1．8，对照组为5．3±1．6、5．6±2．3，两组比较，差异有统计学意义（P〈0．05）；AQP3、9蛋白在研究组绒毛膜滋养细胞的表达强度为7．5±2．0、10．0±1．6，对照组为5．4±2．2、5．6±2．1，分别比较，差异也有统计学意义（P〈0．05），但是AQP3、9蛋白在胎盘滋养细胞的表达强度（3．5±1．4、4．0±2．5）较对照组（5．6±1．3、7．1±2．9）明显下调（P〈0．05）。结论AQP3、9神：特发性羊水过多产妇胎彘和胎膜中的表达变化可能为羊水增加后的代偿性反应，其调节机制尚待进一步研究。Objective To investigate the pathogenesis role of aquaporin 3 and aquaporin 9 in idiopathic polyhydramnios by detecting their expression and distribution in fetal membranes and placenta. Methods Twenty-one of term pregnancy women with idiopathic polyhydramnios were enrolled as patient group matched with 30 women with normal term pregnancy as control group. The expression and localization of aquaporin 3 and aquaporin 9 in fetal membranes and placenta were detected by real-time polymerase chain reaction and streptavidin peroxidase immunohistochemiscal staining. Results （1） The mRNA expressions of aquaporin 3 and aquaporin 9 were detected in amnion, chorion and placental tissue in both patient group and control group. Both aquaporin 3 and aquaporin 9 were demonstrated positive staining in the amnion epithelia, ehorion cytotrophoblasts and placental trophoblast. （2） The ratio of aquaporin 3 and aquaporin 9 mRNA expressions in amnio, in patient group comparing to those in control group were 5.00 and 3.25, while in chorion they were 2.03 and 2. 08. When compared with those in amnion and chorion of control group, there was a significant difference（P 〈 0. 01 ）. However, the relative change fold of aquaporin 3 and aquaporin 9 in placental trophoblast in patient group were decreased in comparison of those in control group, which also showed statistical difference （ P 〈 0. 01 ）. （ 3 ） The expression of aquaporin 3 and aquaporin 9 protein in amnion were 7.5 ±2.0 and 11.1 ± 1.8 in patient group, while they were 5.3 ± 1.6 and 5.6 ± 2. 3 in control group. In chorion, the expression of aquaporin 3 and aquaporin 9 protein was 7.5 ±2.0 and 10. 0 ±1.6 in patient group, respectively, while in control group, they were 5.4 ± 2.2 and 5.6 ±2. 1. When compared with those proteins in control group, it exhibited statistical difference （P 〈 0. 05）. However, in placental trophoblast of patient group, the expression of aquaporin 3 and aquaporin 9 protein were 3.5 ± 1.4 and 4. 0 ± 2. 5, respectively, whi

关 键 词：羊水过多 水通道蛋白质3 水孔蛋白质类 胎盘 胚外膜 

分 类 号：R714.2[医药卫生—妇产科学]