卵泡未破裂黄素化患者子宫内膜中雌、孕激素受体及整合素αVβ3的表达及其意义  

Expression of estrogen receptor, progesterone receptor and integrin αVβ3 in endometrium of patients with luteinized unruptured follicle

作　　者：蒋蕾[1] 徐明[1] 杨菁[1] 谢青贞[1] 敖良飞[1] 

机构地区：[1]武汉大学人民医院生殖医学中心,430060

出　　处：《中华妇产科杂志》2009年第12期929-932,共4页Chinese Journal of Obstetrics and Gynecology

摘　　要：目的研究卵泡未破裂黄素化（LUF）患者子宫内膜中雌激素受体（ER）、孕激素受体（PR）及整合素αVβ3的表达，探讨其对子宫内膜容受性的影响。方法选择2007年5～11月在武汉大学人民医院生殖医学中心拟行冻融胚胎移植的17例发生LUF的不孕患者为LUF组，选择同期排卵周期正常的13例不孕患者作为对照组。于两组患者黄体生成素（LH）峰出现后第7～11天刮取子宫前、后壁获取子宫内膜组织，免疫组化链霉菌抗生物素蛋白-过氧化物酶（SP）连接法检测子宫内膜中ER、PR及整合素αVβ3的表达；同时采用化学发光法测定两组患者血清雌二醇、孕酮水平；分析LUF组患者子宫内膜中整合素αVβ3表达水平与血清雌二醇、孕酮水平的关系。结果（1）LUF组及对照组患者血清雌二醇水平分别为（656±299）和（727±275）pmol／L，两组间比较，差异无统计学意义（P〉0．05）；孕酮水平分别为（23±8）和（35±10）nmol／L，两组间比较，差异有统计学意义（P〈0．01）。（2）LUF组患者子宫内膜中ER及PR的表达水平分别为（183．9±2．4）、（168±3）分，均高于对照组[分别为（109．4±6．3）、（106±4）分]，整合素αVβ3的表达水平为（115±11）分，低于对照组[（191±9）分]，两组间分别比较，差异均有统计学意义（P〈0．01）。（3）LUF组患者子宫内膜中整合素αVβ3的表达水平与血清孕酮水平呈正相关关系（r=0．77，P〈0．01），而与血清雌二醇水平无关（r=0．01，P〉0．05）。结论LUF患者子宫内膜中ER及PR的表达升高，整合素αVβ3的表达降低，可能导致子宫内膜容受性受损，从而影响胚胎的着床。Objective To investigate endometrium receptivity in patients with luteinized unruptured follicle （LUF） by measuring the expression of estrogen receptor （ER）, progesterone receptor （PR） and integrin αVβ3 in the endometrium. Methods From May 2007 to Nov. 2007, 17 infertile women with LUF were selected as LUF group matched with 13 infertile cases with normal ovulation as control group. They all underwent frozen-thawed embryo transfer in Reproductive Medicine Center, Renmin Hospital of Wuhan University. Endometrial tissue in anterior and posterior wall of uterus of LUF group and control group were biopsied by a small curettage between 7 and 11 days after luteinizing hormone （LH） surge. The expression of ER, PR and integrin αVβ3 in endometrium were detected by immunohistochemistry staining. The level of estrogen and progesterone were measured by chemiluminescence assay. Then, the relationship between αVβ3 expression in endometrium and the level of estrogen/progesterone were analyzed in LUF patients. Results （1） There was no remarkable difference in the level of estrogen between LUF [ （656 ± 299 ） pmol/L ] and control group [ （727 ± 275） pmol/L, P 〉 0. 05 ]. However, the level of progesterone were （23 ± 8） nmol/L in LUF group and （35 ± 10） nmol/L in control group,which reached statistical difference （P 〈0. 01 ）. （2） The expression of ER, PR in endometriurn of LUF patients were 183.9 ± 2.4 and 168 ± 3, which were significantly higher than 109.4 ± 6. 3 and 106 ± 4 in control group（ P 〈 0. 01 ）. The expression of integrin αVβ3 in endometrium of 115 ± 11 in LUF group were significantly lower than 191 ±9 in control group（P 〈 0. 01 ）. （4） In LUF group, the expression of αVβ3 in endometrium was correlated positively with the level of progesterone （ r = 0. 77, P 〈 0. 01 ） and irrelevant with the level of estrogen （ r = 0. 01, P 〉 0. 05 ）. Conclusion The higher expression of estrogen and progesterone and lower expression of integrin

关 键 词：无排卵 子宫内膜 受体 雌二醇 受体 孕酮 整合素ΑVΒ3 

分 类 号：R714.8[医药卫生—妇产科学]