有机添加物对卡特兰组织培养的影响  

The Effect of Organic Compounds on the Tissue Culture of Cattleya

作　　者：马生健[1] 覃金芳[1] 曾富华[1] 

机构地区：[1]湛江师范学院生物科学与技术学院,广东湛江524048

出　　处：《中国农学通报》2010年第1期32-35,共4页Chinese Agricultural Science Bulletin

基　　金：广东省科技计划项目(2002A2070402)

摘　　要：以卡特兰(Cattleya labiata)品种MC1原球茎(protocorm-like-body,PLB)为外植体,接种到添加不同浓度(10%、15%、20%)的六种有机添加物(椰乳、椰汁、香蕉泥、马铃薯、水解酪蛋白、苹果汁)的改良VW+5.0mg/L6-BA+0.5mg/LNAA+20g/L蔗糖+16g/L琼脂的培养基上。结果表明:6种有机添加物对PLB增殖的促进作用由大到小依次为椰乳、马铃薯、椰汁、香蕉泥、苹果汁、水解酪蛋白。15%的椰乳对PLB增殖效果最佳,PLB的增殖率最高,增殖期间PLB的分化率也最低。PLB的年龄也影响其增殖作用,年龄越小,增殖率越高,分化率越低。The Cattleya labiata MC1 PLB were inoculated on the reformed VW ＋ 5.0 mg/L 6-BA ＋ 0.5 mg/L NAA ＋ 20 g/L srose ＋ 16 g/L agar medium adding six different organic compounds （Coconut milk、coconut juice、banana mud、Potato、Casein hydrollysate、Apple juice） with different concentrations （10%,15%,20%）. The results showed that, the promotion effect of these six organic compounds from large to small successively is, Coconut milk、Potato、Coconut juice、Banana mud、Apple juice、Casein hydrollysate. The 15% Coconut milk was the best to the propagation of Cattleya PLB, with the highest propagation rate and the lowest differentiation rate. The ages of the PLB also affected the propagation of the PLB. The younger the PLB were, the higher the propagation rate was, and the lower the differentiation rate was.

关 键 词：卡特兰 原球茎 有机添加物 组织培养 增殖 

分 类 号：S3[农业科学—农艺学]