内陆地区自然环境中战备消毒包内细菌同源性的实验研究  

Bacterial homology in sterile packages for war readiness under natural environment in inland region

作　　者：张颖[1] 王仙园[1] 周娟[1] 

机构地区：[1]第三军医大学护理系野战护理学教研室,重庆400038

出　　处：《第三军医大学学报》2010年第1期16-18,共3页Journal of Third Military Medical University

基　　金：全军医学科研"十一五"计划专项课题(06Z078)~~

摘　　要：目的探讨内陆地区自然环境中战备消毒包内细菌来源。方法根据战备消毒包内蜡样芽孢杆菌、藤黄微球菌、科氏葡萄球菌解脲亚种筛选战备仓库空气同种细菌不同菌株,提取基因组DNA,以其为模板,对16S rRNA和23SrRNA的转录间隔区(internal transcribed spacer,ITS)序列进行PCR扩增,将扩增产物回收测序,将测得的基因序列用Blast进行比对。结果蜡样芽孢杆菌、藤黄微球菌、科氏葡萄球菌解脲亚种DNA PCR扩增产物分别在850、1 200、1 000 bp左右显现单一特异性条带;战备消毒包与空气同种细菌不同菌株之间的ITS序列相似性为98%～99%。结论内陆地区自然环境中战备消毒包内细菌来源于空气细菌。Objective To investigate the origin of bacteria in sterile packages for war readiness under natural environment in inland region. Methods Genomic DNAs from the Bacillus cereus, Micrococcus luteus and Staphylococcus cohnii ureal in sterile packages for war readiness and from air bacteria strains in war readiness warehouse were extracted, and then used as templates to amplify the 16S rRNA and 23S rRNA ITS （internal transcribed spacer） sequences by PCR. The PCR products were extracted and sequenced, and the sequences homology analysis was performed with BLAST. Results Amplification products of DNA PCR from Bacillus cereus, Micrococcus luteus and Staphylococcus cohnii ureal were about 800, 1 200 and 1 000 bp respectively; The 16S rRNA-23S rRNA ITS sequences of bacterial strains in sterile packages shared 98% to 99% homology with those from the air bacteria strains in war readiness warehouse. Conclusion The bacteria in sterile packages for war readiness come from the air in war readiness warehouse under natural environment in inland region.

关 键 词：内陆地区 战备消毒包 细菌 同源性 

分 类 号：R821.15[医药卫生—临床医学] R117