质粒 pMC73A 在宿主细菌中的稳定性研究  

Study on Stability of Plasmid pMC73A Harbored in host Bacteria

作　　者：邱晓颖[1] 朱红惠[1] 卢秋雁[1] 丘元盛[1] 

机构地区：[1]广东省微生物研究所

出　　处：《高技术通讯》1998年第6期52-55,共4页Chinese High Technology Letters

基　　金：863计划资助项目

摘　　要：针对基因工程菌在农业生产应用上的安全性问题，对质粒ｐＭＣ７３Ａ在宿主细菌ＮＧ１３中的稳定性进行了研究。试验结果表明：质粒ｐＭＣ７３Ａ在宿主细菌中是不稳定的。在宿主细菌连续繁殖过程中，质粒ｐＭＣ７３Ａ随宿主细菌的繁殖而发生缺失，当宿主细菌繁殖７０代时，质粒ｐＭＣ７３Ａ的缺失超过５０％；宿主细菌繁殖约１２０代，质粒ｐＭＣ７３Ａ的缺失几乎达到１００％。In view of the agricultural application safety of genetic engineered bacteria, the stability of plasmid pMC73A, harbored in Klebsiella oxytoca NG13, was investigated. The results showed that the plasmid pMC73A was unstable in host cells. During continuous propagating of host cells, the plasmid pMC73A loss occurred along with the propagating of host cells. After propagating of host cells for 70 generations, the loss rate of plasmid pMC73A was over 50%, and for 120 generations, the loss rate was almost 100%.

关 键 词：耐氨固氮菌 NIFA基因 质粒稳定性 水稻 固氮菌 

分 类 号：S154.381[农业科学—土壤学] Q788[农业科学—农业基础科学]