血管生成素在糖尿病大鼠肾组织中的表达及氯沙坦的作用  

Effect of Losartan on expression of angiopoietins in the diabetic rats

作　　者：顾红卫[1] 倪兆慧[1] 顾乐怡[1] 严玉澄[1] 戴慧莉[1] 李宁丽[2] 张敏芳[1] 钱家麒[1] 

机构地区：[1]上海交通大学医学院附属仁济医院肾脏科,上海200127 [2]上海交通大学医学院上海免疫研究所,上海200025

出　　处：《中国血液净化》2010年第1期32-36,共5页Chinese Journal of Blood Purification

基　　金：上海市科学委员会基金(014319360);上海交通大学医学院"百人计划"上海交通大学医学院附属仁济医院院基金

摘　　要：目的探讨血管生成素(angiopoietins,Ang)及其受体Tie-2和血管内皮生长因子(VEGF)在糖尿病大鼠肾组织中的表达以及氯沙坦对其表达的影响。方法将SD大鼠随机分为正常对照组(A组),糖尿病组(B组)和糖尿病氯沙坦治疗组(C组),用链脲佐菌素(STZ)诱导大鼠生成糖尿病模型。治疗8周后处死大鼠,采用实时定量RT-PCR和免疫组化方法分别检测三组大鼠肾组织中Ang-1、Ang-2、Tie-2和VEGF的表达;测定大鼠平均肾小球体积和肾小球细胞外基质相对含量;同时检测大鼠尿蛋白/肌酐值等指标。结果与A组大鼠相比,B组大鼠肾组织VEGF和Ang-2表达水平显著升高(P<0.05)。经氯沙坦治疗后,上述血管生长因子表达水平显著下降(P<0.05)。Ang-2表达水平与平均肾小球体积、尿蛋白/肌酐值成正相关(P<0.05)。结论Ang/Tie-2参与早期糖尿病肾损害的发生。氯沙坦可能通过下调这些血管生长因子的表达发挥肾保护作用。Objective To study the expression of angiopoietins（Ang）, Tie-2 and vascular endothelial growth factor（VEGF） in the renal tissue of diabetic rats and the effect of Losartan on their expression. Methods SD rats were randomized into normal control group （group A）, diabetes group （group B） and diabetes treated with Losartan （group C）. Renal expression of VEGF, Ang-1, Ang-2 and their receptor Tie-2, were assessed using real-time reverse transcrip- tion Polymerase chain reaction and immunohistochemistry. Results The expression of VEGF and Ang-2 increased significantly in the renal cortex of group B compared with that in group A （P 〈 0.05）. Ang-2, Tie-2 and VEGF expression of group C decreased significantly compared with group B （P 〈 0.05）. Ang-2 expression correlated positively with mean glomerular volume and urine protein/creatinine ratio （P 〈 0.05）. Conclusion Ang/Tie-2 may play an important role in the development of early diabetic nephropathy, Losartan may exert an renal protection on diabetic nephropathy, possibly partly through the changes of these vascular growth factors expression.

关 键 词：血管生成素 糖尿病 氯沙坦 血管内皮生长因子 

分 类 号：R45[医药卫生—治疗学]