体外非接触共培养诱导骨髓间充质干细胞向肺泡上皮细胞的分化  

Establishment of co-culture model in vitro to induce bone marrow mesenchymal stem cells differentiate into lung epithelial cells

作　　者：王艳[1,2] 杨志军[2] 何玺玉[2] 封志纯[2] 

机构地区：[1]南方医科大学珠江医院儿科,广州510080 [2]北京军区总医院附属八一儿童医院新生儿科,北京100700

出　　处：《西安交通大学学报（医学版）》2010年第1期54-58,105,共6页Journal of Xi’an Jiaotong University（Medical Sciences）

基　　金：国家自然科学基金资助项目(No.30772036)~~

摘　　要：目的建立体外非接触共培养模型,诱导小鼠骨髓间充质干细胞(MSCs)向肺泡上皮细胞的分化。方法分三组,每组6例。对照组:小鼠MSCs单独培养组;实验组A:正常肺组织单细胞悬液+MSCs;实验组B:损伤肺组织单细胞悬液+MSCs。共同培养8 d,激光共聚焦和RT-PCR检测共培养体系下室中的SP-C和AQP5的表达情况。结果对照组和实验组A都只检测到AQP5;而实验组B可同时检测到AQP5和SP-C。实验组B的AQP5 mRNA的表达量较对照组明显增多(P<0.01),而与实验组A比较,其AQP5 mRNA的表达量也有统计学差异(P<0.01)。但是,实验组A与对照组比较则无明显统计学差异(P>0.05)。结论本实验室成功建立了体外非接触诱导小鼠MSCs分化为肺泡上皮细胞的实验模型。Objective To establish the co-culture model in vitro and induce bone marrow mesenchymal stem cells （MSCs） to differentiate into lung alveolar epithelial cells. Methods Each group had 6 samples, control group was MSCs alone; Group A was the MSCs cultured with the cells from normal lung; and Group B was the MSCs with the cells from injuried lung. Each group was cultured for 8 days and the two markers of lung alveolar epithelial cells including AQP5 and SP-C were tested by laser confocal microscopy and RT-PCR. Results Only AQP5 was detected in the control group and Group A, both AQP5 and SP-C were detected in Group B, the AQP5 mRNA expression in Group B was significantly increased compared with that in the control group（P〈0.01）. The AQP5 mRNA expression in Group B was also significantly increased compared with that in Group A （P〈0. 01）. But there was no significant difference in AQP5 mRNA expression between Group A and control group. Conclusion We have successfully established the co-culture model in vitro to induce bone marrow mesenchymal stem cells to differentiate into lung epithelial cells.

关 键 词：骨髓间充质干细胞 肺泡上皮细胞 激光共聚焦 

分 类 号：R725[医药卫生—儿科]