反相流动注射化学发光法测定含巯基药物的含量  

Determination of sulfydryl-containing drugs by reverse flow injection chemiluminescence analysis

作　　者：张广彬[1] 陈宪民[2] 李海燕[1] 于洪玲[1] 孙四娟[1] 唐玉海[1] 

机构地区：[1]西安交通大学理学院分析科学研究所,陕西西安710061 [2]江苏盐城师范学院,江苏盐城224002

出　　处：《西安交通大学学报（医学版）》2010年第1期125-128,共4页Journal of Xi’an Jiaotong University（Medical Sciences）

摘　　要：目的建立一种快速、准确、高灵敏度的检测含巯基药物的化学发光分析方法。方法在硫酸酸性介质条件下,高锰酸钾可与乙二醛产生化学发光,而含巯基药物对该化学发光有显著的增强作用,基于此现象建立了流动注射化学发光分析法测定含巯基药物的新方法。结果实验对影响化学发光的可能因素和参数进行了优化,在优化的条件下,甲巯咪唑、卡托普利和乙酰半胱氨酸分别在1.0×10-8～5.0×10-6、7.0×10-8～1.0×10-6和3.0×10-8～1.0×10-6g/mL浓度范围内与相对化学发光强度成良好的线性关系,检测限分别为1.0、3.9、3.7 ng/mL。结论本方法已成功应用于3种含巯基药物制剂的含量测定,与药典方法比较,结果满意。Objective To establish a rapid, accurate and sensitive chemiluminescence method for determining sulfydryl-containing drugs. Methods In sulfuric acid solution, glyoxal could be oxidized by potassium permanganate, and weak chemiluminescence could be observed. Chemiluminescence signal could be enhanced in the presence of sulfydryl-containing drugs. Thus, on this basis we established a new method of determining the concentration of sulfydryl-containing drugs with flow injection chemiluminescence analysis. Results Under the optimized conditions, the linear range of methimazole, captopril and acetylcysteine was 1.0 × 10^-8 - 5.0 × 10^-6 , 7.0×10^-8 - 1.0 × 10^-6 and 3.0 × 10^-8 - 1.0 × 10^-6 g/mL, respectively. The limit of detection of methimazole, captopril and acetylcysteinc was I. 0, 3.9 and 3.7 ng/mL, respectively. Conclusion The method was successfully applied to determine the three drugs that contain sulfydryl. Compared with the results of pharmacopeia methods, the results we obtained were satisfactory.

关 键 词：流动注射 化学发光 含巯基药物 

分 类 号：R917[医药卫生—药物分析学]