锌指基因ZFP580在大鼠心肌缺血损伤中的表达  

The expression of zinc finger protein 580 in myocardium of rat with ischemic injury

作　　者：孟祥艳[1] 孙慧燕[1] 孔麟麟[1] 张文成[1] 

机构地区：[1]武警医学院生理学与病理生理学教研室,天津300162

出　　处：《武警医学院学报》2010年第1期1-3,F0002,共4页Acta Academiae Medicinae CPAPF

基　　金：国家自然科学基金资助项目(30770885);武警医学院面上项目(WY200804)

摘　　要：【目的】观察大鼠ZFP580基因在急性心肌缺血的不同时期的表达变化,以初步探讨锌指基因ZFP580在心肌缺血损伤中的作用。【方法】腹腔注射异丙肾上腺素(ISO)建立大鼠急性心肌缺血模型,分别于ISO注射后1h、24h取材,对照组腹腔注射等量生理盐水。观察心肌形态学改变,检测血清中乳酸脱氢酶(LDH)及肌酸激酶(CK)的含量,半定量RT-PCR及免疫组化方法分析ZFP580 mRNA及蛋白水平的表达变化。【结果】ISO处理组大鼠心肌组织广泛缺血损伤,血清LDH、CK含量明显升高(P<0.05),心室肌ZFP580mRNA表达下调(P<0.05),免疫组化染色阳性细胞百分率明显降低,以ISO注射1h后变化更为明显。【结论】ZFP580是心肌缺血损伤的早期调控基因之一,其表达下调可能参与了心肌缺血损伤的形成。[ Objective ] To investigate the expression of ZFP580 in myocardium of rat during different periods of myocardial ischemic injury. [Methods] The rat myocardial ischemic injury models were established by intraperitoneal injection of isoprenaline（ISO）. Then the rats with typical electrocardiographic changes were put to death 1 h or 24 h later. The control rats were injected with normal saline as the same dose intraperitoneally. Analyzed the changes of morphology, detected the serum content of creatine kinase （CK） and lactate dehydrogenase （LDH）, measured the expression of ZFP580 mRNA by RT-PCR and evaluated ZFP580 protein expression by immunohistochemical analysis. [Results] Histological sections of ISO-treated rats showed severe myocardial ischemic injury. The contents of CK and LDH in serum of model rats were significantly higher than those of the control rats. Both mRNA and protein levels of ZFP580 in myocardium were also significantly reduced. [Conclusions] ZFP580 is a novel controlling gene related to myocardial ischemic injury and may play an important role in the early period ischemic injury.

关 键 词：锌指基因 ZFP580 异丙肾上腺素 心肌缺血 

分 类 号：R541[医药卫生—心血管疾病]