Effects of Electromagnetic Pulse on Bone Metabolism of Mice in vivo  

作　　者：KANG-CHU LI SHI-RONG MA GUI-RONG DING YAO GUO GuO-ZHEN GUO 

机构地区：[1]Department of Radiation Medicine, Fourth Military Medical University, Xi 'an 710032, Shaanxi China [2]Department of Pathology, Fourth Military Medical University, Xi'an 710032, Shaanxi China

出　　处：《Biomedical and Environmental Sciences》2009年第6期518-521,共4页生物医学与环境科学（英文版）

基　　金：supported by the Research Fund of National Natural Science Foundation of China (No.30800928,No.30670492);the National 863 Project (No.2006 AA02Z4C3)

摘　　要：Objective To study the effects of electromagnetic pulse （EMP） on bone metabolism of mice in vivo. Methods Twenty-four male BALB/c mice were divided into a control group and 2 experimental groups （n=8）. The whole-body of mice in experimental groups were exposed to 50 kV/m and 400kV/m EMP, 400 pulses daily for 7 consecutive days at 2 seconds intervals. Alkaline phosphotase （ALP） activity, serum calcium concentration and osteocalcin level and trabecular bone volume （BV/TV, %） were measured immediately after EMP exposure by biochemical, ELISA and morphological methods. Results The ALP activity, serum calcium concentration and osteocalcin level and BV/TV in experimental groups remained unchanged after EMP exposure. Conelusion Under our experimental conditions, EMP exposure cannot affect bone metabolism of mice in vivo.Objective To study the effects of electromagnetic pulse （EMP） on bone metabolism of mice in vivo. Methods Twenty-four male BALB/c mice were divided into a control group and 2 experimental groups （n=8）. The whole-body of mice in experimental groups were exposed to 50 kV/m and 400kV/m EMP, 400 pulses daily for 7 consecutive days at 2 seconds intervals. Alkaline phosphotase （ALP） activity, serum calcium concentration and osteocalcin level and trabecular bone volume （BV/TV, %） were measured immediately after EMP exposure by biochemical, ELISA and morphological methods. Results The ALP activity, serum calcium concentration and osteocalcin level and BV/TV in experimental groups remained unchanged after EMP exposure. Conelusion Under our experimental conditions, EMP exposure cannot affect bone metabolism of mice in vivo.

关 键 词：Electromagnetic pulse Alkaline phosphotase Serum calcium OSTEOCALCIN Trabecular bone volume 

分 类 号：Q581[生物学—生物化学] TU895[建筑科学]