大豆质核互作雄性不育系W931A及其保持系的差异蛋白质组学比较分析  

Proteomic Analysis of Differentiation Between Cytoplasmic-Nuclear Male-Sterile Line W931A and Its Maintainer in Soybean

作　　者：陈培[1] 张磊[1] 邱丽娟[2] 陆柏[3] 

机构地区：[1]安徽省农业科学院作物研究所,安徽合肥230031 [2]中国农业科学院作物科学研究所,北京100081 [3]安徽经济管理学院培训管理处,安徽合肥230059

出　　处：《作物杂志》2009年第6期17-21,共5页Crops

基　　金：安徽省自然科学基金重点项目(08040102004);国家高技术研究发展计划"863"资助项目(2006AA100104);国家科技支撑计划资助项目(2006AD01A04)

摘　　要：采取SDS-PAGE与LC-MS/MS联用方法,对大豆质核互作雄性不育系W931A及其同型保持系W931B不同器官的蛋白质组进行比较性研究,获得差异蛋白质组的重要信息,并初步分析差异蛋白的主要功能,以期找到与大豆不育性有关的蛋白质,揭示雄性不育发生机理。研究发现,不育系W931A与其保持系W931B种子和苗期叶片的蛋白质SDS-PAGE电泳图谱基本没有差异带,二胞花粉期花药SDS-PAGE图谱间有3条差异带。雄性不育基因表达具有时空和器官特异性,与育性有关的蛋白主要在花药中表达。对差异表达的蛋白如ADP-葡萄糖焦磷酸化酶、NBS-LRR疾病抵抗蛋白质的同源物、ATP合成酶b亚基、硫氧还蛋白过氧化物酶、类锌指蛋白等进行功能分析,推测不育系W931A的雄性不育可能与淀粉合成受抑制,能量代谢紊乱和细胞凋亡有关。The paper was aimed at the comparative proteome analysis of different organs between the cytoplasmicnuclear male-sterile line W931A and its maintainer W931 B in soybeans to obtain some important information and understanding the male-sterile mechanism through analysing the functions of differentially expressed proteins via SDS-PAGE and LC-MS/MS. The SDS-PAGE gel electrophoresis maps showed that no protein spots were found differentially expressed between the male-sterile line W931A and its maintainer W931B seeds or leaves, but 3 protein spots were found differentially expressed between W931 A and W931B anthers at binucleate pollen stage. The above results inferred that the expression of the male sterile genes was spatical- and organ-specific. The functions of the major differentially expressed proteins,including ADP-glucose pyrophosphorylase,NBS-LRR disease resistance protein homologue, ATP synthase, B subunit, thioredoxin-dependent peroxidase and zinc finger protein-like were reviewed and discussed. It was inferred that the male sterility of W931A might be related to starch synthesis,energy metabolism turbulence and cell death.

关 键 词：大豆 质核互作雄性不育 差异蛋白质组学 凝胶电泳 串联质谱 

分 类 号：S565.1[农业科学—作物学]