春甘蓝花芽分化至抽薹过程中生理生化指标的变化  

Changes of Physiological and Biochemical Indexes from Budding to Bolting in Spring Cabbage

作　　者：杨小明[1] 李成琼[1] 宋洪元[1] 任雪松[1] 司军[1] 

机构地区：[1]西南大学园艺园林学院,重庆市蔬菜学重点实验室,重庆400716

出　　处：《中国蔬菜》2009年第24期19-23,共5页China Vegetables

基　　金：重庆市"十一五"科技攻关项目(10376);国家科技支撑计划项目(2006BAD01A7203);国家科技支撑计划(2008BADB1B02-5)

摘　　要：以两个抽薹性不同的春甘蓝材料2006042和春丰为试材,利用石蜡切片法观察甘蓝花芽分化发育的过程,并对不同阶段发育过程中生理生化指标进行分析。结果表明:易抽薹材料2006042花芽分化进程明显早于耐抽薹品种春丰,早8d左右;2006042的可溶性蛋白电泳分析显示在抽薹期出现了3条表达明显增强的特异蛋白,分子表达量分别为48.4、18.8、14.5kD,同时84.0kD条带消失,17.4kD条带减弱,说明这几种蛋白质与抽薹密切相关;易抽薹材料2006042的可溶性蛋白质和蔗糖含量始终比耐抽薹材料春丰高。Taking 2 different spring cabbage （ Brassica oleracea L. ） varieties ‘2006042 ’ and ‘Chunfeng’as materials,the paper observes their development process of flower bud differentiation by petrolin section-cutting method and analyzes the changes in physiological and biochemical indexes during different development stages. The results show that the budding process of bolting susceptible cabbage ‘2006042’is about 8 days earlier than that of the bolting tolerant cabbage ‘Chunfeng’. The analysis of soluble protein in ‘ 2006042 ＇ with SDS - PAGE finds three special protein bands （48.4 kD, 18.8 kD,14.5 kD） are expressively re-enforced, and at the same time the band （84.0 kD） is disappeared and the band （ 17.4 kD） is weakened. This indicates that the above mentioned proteins have very close relation with bohing. The contents of soluble protein content and sucrose in ‘ 2006042 ＇ are higher than that in ‘Chunfeng＇ .

关 键 词：春甘蓝 花芽分化 抽薹 生理生化指标 

分 类 号：S635.1[农业科学—蔬菜学]