新城疫病毒融合蛋白的克隆和序列分析  

Clone and Sequence Analysis of Fusion Gene from NDV

作　　者：原韬[1,2] 赵晓龙[3] 

机构地区：[1]黑龙江省科学院微生物研究所,哈尔滨150010 [2]哈尔滨工业大学,哈尔滨150001 [3]城市水资源开发利用(北方)国家工程研究中心,哈尔滨150090

出　　处：《长春理工大学学报（自然科学版）》2009年第4期625-627,共3页Journal of Changchun University of Science and Technology(Natural Science Edition)

摘　　要：根据NDV-LaSota-F(新城疫病毒LaSota株融合蛋白基因)亚家族氨基酸序列相似性,设计一对NDV-F特异引物,通过RT-PCR方法扩增得到全长1758bp的融合蛋白序列。利用生物信息学软件对此序列进行了开放阅读框分析、同源性分析、氨基酸组成分析、二级、三级结构预测。结果表明,该克隆片段为NDV融合蛋白。One pair of primers was designed to obtain NDV-F subfamily conserved sequence motifs in NDV according to similar features of amino acid sequence in NDV.Amplified the whole fusion protein sequence,about 1758 pb,by RT-PCR.The ORF analysis,homology analysis,hydrohobicity analysis,phospholyration sites analysis,secondary and tertiary struc-ture prediction were carried out to the sequence using bio-information software.The resule showed that the fragment was fus-ion protein in NDV.

关 键 词：NDV LA SOTA 融合蛋白 序列分析 结构预测 

分 类 号：S852.65[农业科学—基础兽医学]