HPLC法测定复方地巴唑氢氯噻嗪胶囊中硫酸胍生和磷酸氯喹的含量  

Determination of Guanoxan Sulfate and Chloroquine Phosphate Hydrochloride in Compound Dibazol and Hydrochlorothiazide Capsules by HPLC

作　　者：陈秀琳[1] 

机构地区：[1]福建省药品检验所,福州350001

出　　处：《中国药品标准》2009年第6期453-455,共3页Drug Standards of China

摘　　要：目的:建立HPLC法测定复方地巴唑氢氯噻嗪胶囊[1]中硫酸胍生[2]和磷酸氯喹[3]的含量。方法:色谱柱为Welch Materials C18柱(4.6 mm×250 mm,5μm),流动相为己烷磺酸钠溶液(取己烷磺酸钠1.35 g,加水适量溶解,加冰醋酸10 mL,并加水至1 000 mL,用三乙胺调节pH至3.5)-甲醇-乙腈(55∶24∶21),流速为1.0 mL.min-1,检测波长为275 nm,柱温为40℃。结果:硫酸胍生在40～200 mg.L-1(r=1.000)范围内线性关系良好,平均回收率为99.3%(n=5),RSD为0.4%;磷酸氯喹在80～400 mg.L-1(r=1.000)范围内线性关系良好,平均回收率为98.7%(n=5),RSD为0.6%。结论:该方法简便、准确、快速,可同时测定两组分的含量。Objective： To establish an HPLC method for the determination of Guanoxan Sulfate and Chloroquine Phosphate in Compound Dibazol and Hydrochlorothiazide Capsules. Methods： The analytical column was Welch Materials C18 column （4. 6 mm ×250mm,5μm）and the mobile phase was a mixture of Sodium Hexanesulfonate solution-methanol-acetonitrile（55： 24：21 ） with flow rate of 1.0 mL ·min^-1. The UV detection wavelength was set at 275 nm. Results： The linearity was good for Guanoxan Sulfate within the range of 40 -200 mg · L^-1 （r = 1. 000） ,the mean recovery was 99. 3% （n =5）, RSD =0. 4% ;The linearity was good for Chloroquine Phosphate within the range of 80 - 400 mg · L ^- 1 （ r = 1. 000 ）, the mean recovery was 98.7% （ n = 5 ）, RSD = 0. 6%. Conclusion： The method is simple and rapid for determination of two components in the drug.

关 键 词：复方地巴唑氢氯噻嗪胶囊 硫酸胍生 磷酸氯喹 高效液相色谱法 

分 类 号：R286.0[医药卫生—中药学] R978.61[医药卫生—中医学]