新城疫病毒弱毒株对鹅的免疫效力试验  

Immune efficacy of attenuated newcastle disease virus strains on geese

作　　者：刘慧谋[1] 胡顺林[1] 孟春[1] 王同燕[1] 王晓泉[1] 刘秀梵[1] 

机构地区：[1]扬州大学兽医学院,江苏扬州225009

出　　处：《中国兽医杂志》2009年第12期14-16,共3页Chinese Journal of Veterinary Medicine

基　　金：国家自然科学基金重大项目(30630048);国家"十一五"科技支撑计划项目(2006BAD06A08)

摘　　要：用新城疫病毒(NDV)疫苗株La Sota和NDV基因Ⅶ型致弱株AND/Ⅶ为免疫原,分别以活苗和灭活苗的形式免疫2周龄试验鹅,免疫后第7、14天和21天采血分离血清用于抗体效价测定。免疫后3周以基因Ⅶ型强毒分离株JS2-06进行攻毒,攻毒后每天观察各组鹅的发病情况,并于第2、4天和7天分别采集喉气管和泄殖腔棉拭样品,用于病毒的分离。试验结果表明,活疫苗免疫后不能产生有效的免疫应答,而灭活苗免疫鹅对强毒的感染具有较强的抵抗能力,但基因Ⅶ型致弱株AND/Ⅶ能更有效抑制鹅体的排毒,其免疫效力明显高于疫苗株La Sota。Two weeks old geese were immunized with La Sota and A-NDV/Ⅶ,an attenuated genotype Ⅶ newcastle disease virus（NDV） strain,respectively.The blood samples were taken on days 7,14 and 21 post-immunization（pi）,and sera were isolated for HI assays.Three weeks pi,the geese were challenged with a lethal dose of a virulent genotype Ⅶ NDV strain.The clinical signs were then observed daily and the swabs were taken from the laryngeal and cloaca on days 2,4 and 7 post-challenge.The morbidity/mortality and virus shedding from the birds showed that both the live vaccines could not elicit effective immune responses and that killed NDV/Ⅶ could provide better immune protection than La Sota in terms of the viral shedding from the vaccinated birds.

关 键 词：新城疫病毒 疫苗 基因Ⅶ型 排毒 

分 类 号：S852.659.5[农业科学—基础兽医学]