3种猪口蹄疫O型油乳灭活疫苗的体液免疫效果比较  

Comparison of Humoral Immune Effects of Three Kinds of Oil-adjuvant Emulsified Inactivated Vaccines of Swine Foot and Mouth Disease Type O

作　　者：司兴奎[1,2] 谢悦机 黄家润 曾宝中 李彦超 

机构地区：[1]佛山科学技术学院动物医学系,佛山528231 [2]广东博罗县温氏畜牧有限公司,博罗516157 [3]广东佛山市南海区狮山镇农林服务中心,佛山528222

出　　处：《中国畜牧兽医》2009年第12期112-115,共4页China Animal Husbandry & Veterinary Medicine

摘　　要：为了确定3种猪口蹄疫O型油乳灭活疫苗的体液免疫效果,将91头51日龄四元杂交猪随机分为7组（13头/组）,除1组作为健康对照组外,AA、BB及CC分别为A、B及C 3种油乳灭活疫苗的二次免疫组,AAA、BBB及CCC为上述3种疫苗的3次免疫组,在首免或二免后4周加强免疫,采用阻断ELISA方法检测首免后2、4、6、8、10及12周（WPI）特异性抗体的阻断率。研究结果表明,在8WPI,AA、BB及CC组抗体阻断率达到峰值,分别为60.1%、39.0%及45.2%,随后抗体水平迅速下降,在12WPI,AA、BB及CC组抗体阻断率分别降至19.7%、25.2%及31.0%;AAA、BBB及CCC组疫苗免疫的6组在8WPI后抗体阻断率呈现上升趋势,在12WPI,AAA、BBB及CCC组抗体阻断率分别为46.3%、48.1%及50.1%,且在10～12WPI抗体阳性率始终维持在46%以上;综上,6组疫苗免疫效果的次序为AA〉CCC〉BBB〉CC〉AAA〉BB。To compare the humoral immune effects of three kinds of oil-adjuvant emulsified inactivated vaccine,ninety one pigs of 51-day-old were randomly divided into healthy control group or AA,BB,CC,AAA,BBB and CCC groups which were inoculated with A,B or C vaccine with two or three times,and booster inoculation was conducted every 4 weeks post inoculation（WPI）.Blocking ELISA was applied to detect specific antibody blocking rates at 2,4,6,8,10 and 12 WPI,respectively.The results showed that the antibody blocking rates of inoculated group AA,BB and CC were peaked at 8WPI,i.e.60.1%,39.0% and 45.2%,and antibody levels were declined sharply since 8 WPI,the corresponding antibody blocking rates were 19.7%,25.2% and 31.0%,respectively;while the antibody blocking rates of AAA,BBB and CCC had been increased gradually since 8 WPI,the corresponding antibody blocking rates were 46.3%,48.1% and 50.1%,and antibody positive rate of each one were no less than 46%,taking together,the humoral immune effects order of six inoculation group was 46.3%,48.1% and 50.1%.

关 键 词：猪 口蹄疫O型 油乳灭活疫苗 体液免疫效果 

分 类 号：S852.4[农业科学—基础兽医学]