猪繁殖与呼吸综合征病毒安徽株的分离与鉴定  

Isolation and Identification of Porcine Reproductive and Respiratory Syndrome Virus from Anhui

作　　者：朱良强[1] 王非[1] 占松鹤[1] 何长生[1] 王爱萍[2] 姚雪静[2] 王立[1] 刘华[1] 王军[1] 沈艳[1] 

机构地区：[1]安徽省动物疫病预防与控制中心,合肥230022 [2]安徽农业大学,合肥230001

出　　处：《中国畜牧兽医》2009年第12期115-119,共5页China Animal Husbandry & Veterinary Medicine

基　　金：安徽省科技攻关项目(0810302150)

摘　　要：从安徽地区的发病猪场的病料中,分离到3株致Marc-145细胞病变的病毒（命名为AH1、AH2、AH3）,负染电镜观察结果可见直径约50 nm的有囊膜的球形病毒粒子,细胞超薄切片可见病毒粒子分散在细胞浆内,直径为40～80 nm。理化特性研究结果表明,3株分离毒均对氯仿、乙醚、甲醛、温度（56℃）敏感;间接免疫荧光试验结果显示,分离株与美洲型PRRS阳性血清反应,发出强特异性荧光;对分离毒株Nsp2基因序列分析发现：安徽分离毒株Nsp2基因存在两处共90个碱基缺失现象,与JXA1 PRRSV变异株亲缘性最近。初步鉴定该分离株为高致病性猪繁殖与呼吸综合征病毒。Three virus strains（AH1,AH2 and AH3） with cytopathic effects were isolated from the invasive swine farms in Anhui area.The virus was sphere with envelope and diameter of 50 nm by negative stain under electron microscope.Thin section showed the virus replicated in cytoplasm with diameter of 40-80 nm.The physicochemical characterization showed these strains were sensitive to the treatment with chloroform,ether,formalin,and 56 ℃.The specific fluorescent were seen when the virus reacted with PRRSV antiserum.There are two gene absence place of 90 nt in Nsp2 gene through gene sequencing.Comparing with the variant JXA1 stain,the isolated virus has the most recent relationship.These results confirmed the isolated virus were high pathogenic porcine reproductive and respiratory syndrome.

关 键 词：猪繁殖与呼吸综合征病毒 分离 鉴定 

分 类 号：S852.65[农业科学—基础兽医学]