化学发光直接竞争免疫分析法检测猪尿中莱克多巴胺残留  

Determination of Ractopamine Residues in Swine Urine by Direct Competitive Chemiluminescence Immunoassay

作　　者：高彬文[1,2] 张素霞[2] 梁永红[1] 刑保松[1] 

机构地区：[1]河南农业科学院畜牧兽医研究所,郑州450002 [2]中国农业大学动物医学院,北京100193

出　　处：《中国畜牧兽医》2009年第12期200-203,共4页China Animal Husbandry & Veterinary Medicine

摘　　要：本试验研究建立了猪尿中莱克多巴胺（Ractopamine,RAC）残留的直接竞争化学发光免疫检测方法。合成了莱克多巴胺和辣根过氧化物酶的偶联物（RAC-HRP）,方法的检测限为0.09 ng/mL,空白猪尿中添加浓度为0.5、10和100 ng/mLRAC时,检测范围为0.3～157.0 ng/mL;回收率为76.3%～87.6%,批内变异系数为10.0%～12.2%,批间变异系数为14.2%～15.2%。A method was developed for the determination of RAC residues in swine urine by direct competitive chemiluminescence immunoassay（CLIA）.The limit of detection was 0.09 ng/mL and the detection ranged from 0.3 ng/mL to 157.0 ng/mL.At the fortified levels of 0.5 ng/mL,10 ng/mL and 100 ng/mL,mean recoveries ranged from 76.3% to 87.6%.The intra-assay variations were 10.0% to 12.2% and the inter-assay variations were 14.2% to 15.2%.

关 键 词：化学发光免疫分析 莱克多巴胺 猪尿 

分 类 号：S859.84[农业科学—临床兽医学]