不同鸡种GARS-AIRS-GART基因21号外显子单核苷酸序列多态性分析  

Single Nucleotide Polymorphism Sequence Analysis of Exon 21 in GARS-AIRS-GART Gene in Different Kinds of Chickens

作　　者：龚琳琳[1] 周琼[1] 廖菁[1] 王存波[1] 常国斌[1] 陈国宏[1] 侯庆文[2] 王伟 

机构地区：[1]扬州大学动物科学与技术学院,江苏扬州225009 [2]淮安市畜牧水产局,江苏淮安223001 [3]苏州市吴中区畜牧水产局,江苏苏州215128

出　　处：《中国家禽》2009年第24期13-16,共4页China Poultry

基　　金：国家十一五863重点项目(2008AA101009);国家十一五科技支撑计划重点项目(2008BADB2D07);现代肉鸡产业技术体系建设专项资金

摘　　要：以安卡鸡、文昌鸡、如皋草鸡为实验材料,采用PCR-SSCP法对GARS-AIRS-GART基因的21号外显子进行SNPs检测。结果发现4个核苷酸多态位点:G29943C、C29968T、T30011C、C30017T,其中3处为非同义突变,分别为:29943处天冬氨酸(Asp)→天冬酰胺(Asn)、30011处色氨酸(Trp)→精氨酸(Arg)、30017处精氨酸(Arg)→半胱氨酸(Cys);另1处为同义突变。对多态位点进行单倍型分析,发现12种单倍型,有3种单倍型的频率超过10%,其余9种都小于10%。另外共检测到11种基因型,存在5种等位基因,安卡鸡的D和E等位基因的基因频率较高,基因型频率与其他2个鸡品种之间都存在着极显著的差异(P<0.01),表明我国地方鸡种和外来鸡种在遗传组成上存在着差异。Single nucleotide polymorphisms （SNPs） of the exon 21 in GARS-AIRS-GART gene in Anka chickens, Wenchang and Rugao chickens were studied with PCR-SSCP. We detected 3 non-synonymous mutations in exon 21, G29943A,T30011C,and C30017T,and the corresponding amino acid changed from aspartic （Asp）,tryptapham（Trp）,and arginine （Arg） to asparagine （Asn）, arginine （Arg）, and cysteine （ Cys ）, respectively, 1 SNPs （ C29968T ） was synonymous. In these chickens,12 haplotypes were reconstructed. The frequencies of 3 haplotypes were more than 10%,the rest of haplotypes less than 10%. Moreover, I1 genotypes and 5 alleles were tbund in three breeds. The D,E alleles of Anka chickens were relatively high,which genotype was significanly different （P〈0.01） from that of other two species. It indicates that Chinese local chicken breeds are different from alien species in genetic composition.

关 键 词：鸡 GARS-AIRS—GART基因 外显子21 单核苷酸多态性 单倍型 

分 类 号：S852.65[农业科学—基础兽医学] Q347[农业科学—兽医学]