长链CpG寡脱氧核苷酸DNA重组质粒对禽流感灭活疫苗免疫应答的影响  

Effects of Recombinant Plasmids Containing Long Strand CpG Oligodeoxynucleotides DNA on Immune Responses of Inactivated Avian Influenza Vaccine

作　　者：司兴奎[1] 陈建红[1] 张济培[1] 

机构地区：[1]佛山科学技术学院动物医学系,广东佛山528231

出　　处：《中国家禽》2009年第24期29-31,35,共4页China Poultry

基　　金：广东省科技攻关项目资助(2004A2090103)

摘　　要：为明确导入长链CpG寡脱氧核苷酸3个DNA重组质粒对禽流感H5亚型灭活疫苗的免疫应答的影响,将21日龄试验雏鸡随机分为6组,即健康对照组(A组)、疫苗对照组(B组)、空载体对照组(C组)、pUC26免疫促进组(D组)、pUC30免疫促进组(E组)及pUC44免疫促进组(F组),对6组雏鸡在首免后1～8周(WPI)检测其HI抗体水平并在1、4及8WPI测定脾脏、法氏囊及胸腺免疫器官指数。研究结果表明:在2WPI,D(P<0.01)、F(P<0.01)及E组(P<0.05)HI抗体水平极显著或显著高于C组;在3WPI,F组HI抗体水平分别显著高于B(P<0.05)、C(P<0.05)及E组(P<0.05);在5WPI,F(P<0.01)和D(P<0.05)组HI抗体水平极显著或显著高于C组;在6WPI,F组HI抗体水平显著高于C组(P<0.05);在8WPI,F组HI抗体水平极显著高于B(P<0.01)或C组(P<0.01)。在4WPI,A组胸腺指数极显著或显著高于C(P<0.05)及D组(P<0.01),E组胸腺指数显著高于D组(P<0.05);在8WPI,E组脾脏指数显著高于A组(P<0.05)。To clarify the immunological enhancement effects of three recombinant plasmids contain long strand CpG oligodeoxynucleotides DNA to recombinant avian influenza vaccine （H5N1 ,Re-1 ）,21-day-age chicken were randomly divided into 6 groups i. e A （healthy control）,B （vaccine control）,C （vacant vector and vaccine control） ,D （pUC26 enhancement group）,E（pUC30 enhancement group）and F（pUC44 enhancement group）. The sera samples of each group were collected at l to 8 weeks post the first immunization（WPI）,and spleen,bursa and thymus indexes were obtained at 1,4 and 8 WPI. The results showed that HI antibody titers of group D （P〈0.01）,F （P〈0.01）and E （P〈0.05）were significantly higher than those of group C at 2 WPI;and HI antibody titers of group F were statistically higher than those of group B（P〈0.05）,C（P〈0.05）and E（P〈0.05）at 3 WPI,respectively;and HI antibody titers of group F（P〈0.01）and D （P〈0.05）were significantly higher than those of group C at 5 WPI;and HI antibody tlters ot group t＇ were statistically higher than those of group C （P〈0.05） at 6 WPI;and HI antibody titers of group F were statistically higher than those of group B （P〈0.0l）and group C （P〈0.01）at 8 WPI. For thymus indexes,group A were significantly higher than group C（P〈0.05）and group D（P〈0.01）at 4WPI,group E were statistically higher than group D（P〈0.05）at 4 WPI, group E were statistically higher than group A （P〈0.05） at 8 WPI.

关 键 词：CpG寡脱氧核苷酸DNA重组质粒 禽流感灭活疫苗 免疫应答 免疫器官指数 

分 类 号：S852.4[农业科学—基础兽医学] S859.797[农业科学—兽医学]