芍药苷增高小鼠恶唑酮结肠炎黏膜中β-防御素表达并减轻病变  

Paeoniflorin increases β-defensin expression and attenuates lesion in the colonic mucosa from mice with oxazolone-induced colitis

作　　者：周毅[1] 王赫[2] 李悦山[1] 陶移文[1] 张建业[1] 张智强[1] 

机构地区：[1]广州医学院药学系,广东广州510182 [2]南方医科大学肿瘤研究所,广东广州510515

出　　处：《药学学报》2010年第1期37-42,共6页Acta Pharmaceutica Sinica

基　　金：广州医学院自然科学基金资助项目(0703012)

摘　　要：观察芍药苷对恶唑酮诱导的实验性结肠炎(IBD)小鼠的结肠组织黏膜中β-防御素-2(HBD-2)、IL-6和IL-10表达的影响。通过建立BALB/c小鼠恶唑酮结肠炎模型,并将其分为正常对照组、恶唑酮诱导组、5-氨基水杨酸(5-ASA)组、芍药苷(20、10及5mg·kg-1)治疗组;进行疾病活动指数(DAI)和组织学评分(HGC);使用免疫组织化学、实时荧光定量逆转录聚合酶链反应(RT-PCR)检测结肠黏膜中HBD-2和细胞因子IL-10、IL-6蛋白及其mRNA的表达。结果显示,恶唑酮诱导组的DAI和HGC显著高于正常对照组(P<0.01),芍药苷各组和5-ASA组的评分均显著低于恶唑酮诱导组(P<0.05,P<0.01);恶唑酮诱导组的HBD-2和IL-6表达较正常对照组均显著升高,且与恶唑酮结肠炎症DAI和HGC呈正相关(Pearsonr=0.728(DAI/HBD-2),Pearsonr=0.758(DAI/IL-6),P<0.01,respectively;Pearsonr=0.819(HGC/HBD-2),Pearsonr=0.825(HGC/IL-6),P<0.01,respectively);恶唑酮诱导组的IL-10表达较正常对照组显著降低(P<0.01),且与恶唑酮结肠炎症DAI和HGC呈负相关(Pearsonr=-0.789(DAI/IL-10),Pearsonr=-0.725(HGC/IL-10),P<0.01,respectively);芍药苷各组和5-ASA组均能显著下调恶唑酮诱导组结肠黏膜部位HBD-2、IL-6含量和上调IL-10蛋白和mRNA的表达。结果表明,芍药苷通过调节HBD-2、IL-6和IL-10的表达来减轻或改善恶唑酮结肠炎小鼠的症状。Previous studies have demonstrated that the Chinese medicine paeoniflorin, derived from the Ranunculaceae plant peony, peony, purple peony root, was able to have anti-inflammatory, anti-ulcer, anti- hypersusceptibility and anti-oxidation activity. In order to elucidate the pesticide effect and the mechanisms by which paeoniflorin exerts its effect of anti-inflammation and immunoregulation on oxazolone-induced colitic mice, disease activity index （DAI） and histological grading of colitis （HGC） were evaluated in animal model. Moreover, the expressions of HBD-2, IL-6 and IL-10 of mice with experimental colitis were observed with immunohistochemistry and RT-PCR in this study. Results showed that DAI and HGC of oxazolone control group was significantly higher than that of normal control group, and that paeoniflorin groups and 5-ASA group, compared with oxazolone control group, could alleviate the symptoms and histological damages of colitic mice （P〈0.05, P〈0.01）. The expression of HBD-2 and IL-6 cytokine on the colon of colitic mice was higher than that of normal control, paeoniflorin and 5-ASA groups （P〈0.05, P〈0.01）, but the expression of IL-10 is lower than that of normal control, paeoniflorin and 5-ASA groups （P〈0.05, P〈0.01）. The positive correlationswere demonstrated between the expression of （HBD-2 and IL-6） and DAI （Pearson r = 0.728, Pearson r = 0.758, P〈0.01, respectively）, （HBD-2 and IL-6） and HGC （Pearson r = 0.819, Pearson r = 0.825, P〈0.01, respectively）, whereas, the negative correlations were demonstrated between the expression of IL-10 and DAI （Pearson r = -0.789, P〈0.01）, IL-10 and HGC （Pearson r = -0.725, P〈0.01）. It can be concluded that to some extent paeoniflorin effectively alleviate the symptoms of oxazolone-induced colitis through regulating the expression of HBD-2, IL-6 and IL-10.

关 键 词：芍药苷 Β-防御素 IL-6 IL-10 恶唑酮结肠炎 

分 类 号：R285[医药卫生—中药学]