桑叶中的黄酮类化合物  

Flavonoids from the leaves of Morus alba L.

作　　者：杨燕[1] 王洪庆[1] 陈若芸[1] 

机构地区：[1]中国医学科学院、北京协和医学院药物研究所,中草药物质基础与资源利用教育部重点实验室,北京100050

出　　处：《药学学报》2010年第1期77-81,共5页Acta Pharmaceutica Sinica

基　　金：科技部基础性工作专项资助项目(2007FY130100)

摘　　要：为了研究桑叶的化学成分与生物活性之间的相关性,采用硅胶、Sephadex LH-20、RP-C18等色谱方法分离纯化,通过核磁共振谱、质谱等波谱分析手段鉴定化合物结构。从长穗桑的95%乙醇提取物中分离到4个Diels-Alder类加合物,分别鉴定为mulberrofuran F1(1)、mulberrofuran F(2)、chalcomoracin(3)和kuwanon J(4);2个查耳酮类化合物,鉴定为morachalcone A(5)和isobavachalcone(6);3个黄酮类化合物,鉴定为norartocarpetin(7)、kuwanonC(8)和6-geranylapigenin(9)。化合物1和6为首次从该种植物中分离,化合物4～5、7～9为首次从桑叶中分离得到,其中化合物1为新化合物。采用MTT法对化合物1～5进行了抗肿瘤活性筛选,结果显示化合物1～3对人肿瘤细胞A549、Bel7402、BGC823、HCT-8以及A2780具有抑制作用。The investigation on the leaves of Morus alba L. was carried out to find the relationship of the constituents and the pharmacological activities. The isolation and purification were performed by various chromatographies such as silica gel, Sephadex LH-20, RP-C18 column chromatography and so on. Further detailed investigation on the fraction of the ethanol extract of leaves of Morus alba yielded four Diels-Alder type adducts mulberrofuran F1 （1）, mulberrofuran F （2）, chalcomoracin （3）, kuwanon J （4）, together with two chalcones morachalcone A （5）, isobavachalcone （6）, and three flavones norartocarpetin （7）, kuwanon C （8）, 6-geranylapigenin （9）. Their structures were elucidated by the spectral analysis such as NMR, MS etc. Compounds 1, 6 were isolated from this plant for the first time, compounds 4–5, 7–9 were isolated from the leaves of Morus alba L. for the first time, among which 1 was a new compound. Compounds 1–5 were evaluated for the cytotoxicity against A549, Bel7402, BGC823, HCT-8 and A2780 cell lines in vitro by MTT method, but only compounds 1–3 showed cytotoxicity against several human cancer cell lines.

关 键 词：桑属 桑叶 抗肿瘤活性 黄酮类化合物 

分 类 号：R284.1[医药卫生—中药学]