硝态氮对淹水条件下甜樱桃根系呼吸速率及相关酶活性的影响  

Effects of NO_3^- on respiratory rate and enzyme activities of roots of sweet cherry under waterlogging conditions

作　　者：王闯[1,2] 胡艳丽[1] 高相彬[1] 沈向[1] 郝云红[1] 毛志泉[1] 

机构地区：[1]作物生物学国家重点实验室山东农业大学园艺科学与工程学院,山东泰安271018 [2]聊城职业技术学院,山东聊城252000

出　　处：《植物营养与肥料学报》2009年第6期1433-1438,共6页Journal of Plant Nutrition and Fertilizers

基　　金：山东省良种工程项目;国家‘十一五’科技支撑重点项目(2008BADA4B05)资助

摘　　要：以盆栽美早/考特甜樱桃(Prunus avium L.)为试材,测定了在0、10、20、30 mmol/L NO3-溶液中甜樱桃根系呼吸速率和相关酶活性的变化。结果表明,NO3-减缓了淹水过程中甜樱桃根系呼吸速率的下降幅度,且随NO3-浓度的升高其呼吸速率加强。淹水23~d后,NO3-处理的植株根系糖酵解途径(EMP)、磷酸戊糖途径(PPP)和三羧酸循环途径(TCA)的呼吸速率均高于对照,同时增强了根系中磷酸果糖激酶(PFK)、葡萄糖-6-磷酸脱氢酶(G-6-PDH)和苹果酸脱氢酶(MDH)的活性。NO3-的加入可以减轻甜樱桃因淹水带来的危害。The objective of this study is to examine the effects of NO3^- on the respiratory rate and its key enzyme activities of roots of sweet cheery （ Prunus avium L. ） under waterlogging. Pot experiments were conducted to determine the respiration and key enzyme activities by using two-year-old Meizao/Colt sweet cherries which were transferred into the solutions with 0, 10, 20 and 30 mmol/L KNO3 for 5 days. The decrease of respiratory rate is alleviated by adding exogenous NO3^- under waterlogging, and respiratory rate of root tissues of sweet cherry could be enhanced when NO3^- concentration is increased. After the waterlogging for 2-3 days, the addition of NO3^- promotes the increases of respiratory rates of Embden- MeyerhofParnas pathway （EMP）, pentose phosphate pathway （PPP） and tricarboxylic acid cycle（TCA）, and as well as the enhancements of enzyme activities of pyrophosphate-dependent phosphofructokinase （PFK）, glucose-6-phosphate dehydrogenase （G-6-PDH） and malate dehydrogenase （MDH）. The addition of NO3^- could lessen the waterlogging damage of the sweet cherry.

关 键 词：硝态氮 淹水 甜樱桃 根系 呼吸速率 酶活性 

分 类 号：S662.5[农业科学—果树学]