咳喘三伏贴中芥子碱硫氰酸盐的超声提取工艺  

Study on the ultrasonic extraction process for sinapine thiocyanate from Kechuansanfutie

作　　者：黄云[1] 张慧芬[1] 王文喜[2] 王珏[1] 毛军燕 

机构地区：[1]浙江大学医学院附属儿童医院,浙江杭州310003 [2]浙江工业大学药学院,浙江杭州310032

出　　处：《中国医院药学杂志》2010年第1期23-25,共3页Chinese Journal of Hospital Pharmacy

基　　金：浙江省中医药科技计划项目资助(编号:2007CA076);浙江省医学会临床科研基金项目(编号:2007ZYC21)

摘　　要：目的:优选咳喘三伏贴处方中芥子碱硫氰酸盐的超声波提取工艺。方法:采用HPLC梯度洗脱法测定芥子碱硫氰酸盐的含量,以芥子碱硫氰酸盐的提取率为指标,通过正交试验优化超声波提取的工艺。结果:最佳提取工艺为混合药材用35倍的60%乙醇提取2次,超声频率为28kHz。结论:优选的工艺提取效率高,稳定可行。OBJECTIVE To optimize the ultrasonic extraction process of sinapine thiocyanate from Kechuansanfutie formula. METHODS Sinapine thiocyanate was determined by HPLC with gradient elution, and the optimal extracting process was ob taincd via an orthogonal test method according to the extracting efficiency of sinapine thiocyanate. RESULTS The optimal ex tracting process was adding 60% alcohol thirty five times as the total weighty of Chinese materials and extracting for two times with 28 kHz ultrasound. CONCLUSION The extracting rate is high, and the process is stable.

关 键 词：芥子碱硫氰酸盐 超声提取 正交设计 

分 类 号：R944[医药卫生—药剂学]