复健片对大鼠脑梗死不同时间点OMgp表达的影响  

Effect of Fujian tablets on expressions of OMgp at different stages in rats with cerebral infarction

作　　者：胡怀强[1] 周永红[2] 马学盛[3] 王新陆[3] 曹秉振[1] 

机构地区：[1]中国人民解放军济南军区总医院神经内科,山东济南250031 [2]青岛大学医学院,山东青岛266071 [3]山东中医药大学,山东济南250355

出　　处：《山东中医药大学学报》2010年第1期64-66,共3页Journal of Shandong University of Traditional Chinese Medicine

基　　金：国家自然科学基金项目(编号:30672745);山东省科学技术发展计划项目(编号:2006GG3202005)

摘　　要：目的:观察复健片在不同时间点对脑梗死大鼠脑组织少突胶质细胞髓鞘糖蛋白(OMgp)表达的影响,探讨其促进神经发生的作用机制。方法:240只Sprague-Dawley大鼠随机分为正常对照组、假手术组、模型组和药物组四个大组,并根据脑梗死病程每组又分为3d、1周、2周、4周、6周5个亚组,每个亚组动物数为12只。用改良的LongaEZ法制备大脑中动脉阻塞大鼠模型,药物组灌胃给予复健片水溶液,余各组分别灌胃给予同剂量的蒸馏水。用免疫组织化学法观察各组大鼠梗死周边区脑组织OMgp的表达。结果:模型组大鼠脑内的OMgp在梗死后即开始表达,逐渐增高,2周时达到高峰,第6周时还处于高表达状态,明显高于正常对照组(P<0.01)。药物组中OMgp表达与模型组比较均有显著性差异(P<0.05)。结论:复健片可抑制OMgp的表达,从而促进神经发生。Objective：To explore the mechanism of Fujian tablets promoting neural regeneration by observing the expressions of oligodendrocyte myelin glycoprotein（OMgp） in rats with cerebral infarction at the different stages. Methods：240 Sprague-Dawley rats were randomly divided into normal control,sham operation,model and medicine groups, which were randomly divided into five subsets with 12 rats according to the 3 d,1 w,2 w,4w and 6w stages. The rat models with middle cerebral artery occlusion were successfully established by the improved Longa EZ. The medicine group were given with Fujian tablets,other group were given with commensurability distilled water. The expessions of OMgp in the region around the infarction were observed by immunohistochemieal method. Results：The OMgp appeared after the cerebral infarction in the model group,and increased gradually,achieved the peak at 2 w,still were in high at 6w,the differences of model compared with the normal control were significant at the same stage（P〈0.01）. The differences of OMgp between medicine group and model group were significant（P〈0.01） at all stages. Conclusion：Fujian tablets could promote neurogenesis by inhibitting the expressions of OMgp.

关 键 词：复健片 脑梗死 神经再生 抑制因子 少突胶质细胞髓鞘糖蛋白 大鼠 

分 类 号：R285.5[医药卫生—中药学]