磷霉素与氨基糖苷类药物联用对体外铜绿假单胞菌生物被膜中活菌数的测定  

The Effect of the Combination of Fosfomycin and Aminoglycosides on the Viable Counts of Pseudomonas Aeruginosa Biofilm in Vitro

作　　者：范燕[1] 王睿[2] 

机构地区：[1]国家食品药品监督管理局药品评价中心,北京100045 [2]解放军总医院临床药理研究室,北京100853

出　　处：《中国药物警戒》2010年第1期1-4,共4页Chinese Journal of Pharmacovigilance

基　　金：国家自然科学基金资助项目(30070720)

摘　　要：目的通过研究磷霉素与氨基糖苷类药物(阿米卡星、异帕米星)联合应用对铜绿假单胞菌生物被膜的体外联合效应,探讨磷霉素对氨基糖苷类药物在抑制铜绿假单胞菌生物被膜形成方面的作用。方法采用改良的组织培养平板法建立铜绿假单胞菌生物被膜模型,应用活菌计数法观察经不同剂量的两类药物单用与联合作用处理后的铜绿假单胞菌生物被膜的活菌数量,对其进行比较与分析。结果联药组处理的生物被膜内活菌数较单药组显著下降,表明磷霉素与阿米卡星/异帕米星二者联合应用后,生物被膜的形成被抑制,存活细菌数量显著减少。结论研究发现磷霉素与氨基糖苷类药物联用对铜绿假单胞菌生物被膜形成的抑制具有显著增效作用。Objective To study the combination effect of fosfomycin and arninoglycosides on the for mation of biofilm of Pseudomonas aeruginosa（PAO1 ） in vitro. Methods The modified plate culture method was used to establish biofilm model in vitro with which the morphology of PAO1 biofilms were observed by the viable counts after treated with different dosages of fosfomycin and aminoglycosides. Results MTT method showed that the viable counts of bacteria of combination gruops were significantly lower than the non-combination groups （P〈0.05）. Conclusions There were obvious differences between combination fosfomycin and aminoglycosides groups and non-combination groups in the formation of PAO1 biofilm. Synergistic action of fosfomycin to aminoglycosides is verified.

关 键 词：磷霉素 氨基糖苷类药物 细菌生物被膜(BBF) 铜绿假单胞菌野生菌株(PAO1) 

分 类 号：R965[医药卫生—药理学]