神经生长因子对糖尿病大鼠背根神经节神经细丝蛋白变化的影响  

The effects of nerve growth factor on the changes of neurofilament in the dorsal root ganglia of diabetic rats

作　　者：贾军宏[1,2,3] 马学毅[1,2,3] 田东华 于国平[1,2,3] 杨毅 王会信[1,2,3] 赵明[1,2,3] 

机构地区：[1]解放军304医院内分泌科 [2]解放军总医院神经内科 [3]军事医学科学院基础所八室

出　　处：《军医进修学院学报》1998年第4期259-261,共3页Academic Journal of Pla Postgraduate Medical School

摘　　要：目的：观察神经生长因子（ＮＧＦ）对糖尿病神经病变的治疗作用。方法：采用免疫组织化学染色结合电子计算机图像分析技术，测定糖尿病大鼠背根神经节的结构蛋白－神经细丝蛋白（ＮＦ）的相对含量。结果：糖尿病成模后三个月，背根神经节ＮＦ的平均灰度值，糖尿病组（ＤＭ组）明显低于正常对照组（ＮＣ组），ＮＧＦ组（ＮＧＦ２００μｇ·ｋｇ－１·ｄ－１肌肉注射，３０ｄ）虽低于ＮＣ组，但却高于ＤＭ组，Ｐ均＜０．０１。结论：ＮＧＦ可促进感觉神经结构蛋白的表达。Objective:To observe the threapeutic effects of nerver growth factor (NGF) on diabetic peripheral neuropathy.Methods:The changes of the neurofilament (NF) in the dorsal root ganglia(DRG) of diabetic rats were measured by the techniques of immunohistochemistry combing with computerized image analysis system. Results:After inducing the diabetes for three months, the mean gray levels (M.G.L.) of the positive reaction of NF in the DRG were much lower in the DM group than in the normal control group, those in the NGF group (NGF 200 μg·kg -1 ·d -1 IM.×30 d) were lower than in normal control group too, but were higher than in DM group ( P <0.01).Conclusion:The NGF may promote the expression of the NF in sensory nerve and seems to have the beneficial effects on the recovery of diabetic peripheral neuropathy.

关 键 词：神经生长因子 糖尿病 背根神经节 神经细丝蛋白 

分 类 号：R587.105[医药卫生—内分泌]