月季RcHSP17.8基因表达提高大肠杆菌对非生物胁迫的耐性  

The Expression of RcHSP17.8 Gene Improving Tolerance to Abiotic Stress in Recombinant E.coli Cells

作　　者：蒋昌华[1,2] 胡永红[1] 徐健遥[2] 张浩[2] 石金磊[2] 李敏[2] 王雅琼[2] 明凤[2] 

机构地区：[1]上海植物园,上海200231 [2]复旦大学生命科学学院 遗传工程国家重点实验室,上海200433

出　　处：《园艺学报》2009年第12期1791-1798,共8页Acta Horticulturae Sinica

基　　金：上海市农委科技兴农重点攻关项目[沪农科攻字(2008)第10-4号];国家‘863’项目(2008AA10Z116);上海市青年科技启明星计划项目(08QH14003);上海市科委重点科技攻关项目(08391910400);国家基础科学人才培养基金项目(J0630643)

摘　　要：月季RcHSP17.8为编码小分子热激蛋白基因,Southern检测其在月季基因组中为多拷贝存在。38℃/3h热激处理后,该基因在耐热月季品种‘曼海姆宫殿’(Schloss Mannieim)、‘彩虹’(Radio)、‘赌城’(Las Vegas)、‘梅郎随想曲’(Capricede Meiland)、‘小女孩’(Girl)中强表达,而在不耐热品种‘新十全’(Kordes Perfecta)、‘绯扇’(Hiogi)、‘莱茵黄金’(Pfalzer Gold)中弱表达或不表达,表明该基因与月季耐热性关系密切。为确定月季RcHSP17.8基因功能,将其转化E.coliBL21,结果表明重组菌株能正常诱导表达包含RcHSP17.8的融合蛋白,并因此提高了重组菌株对高温、低温、高盐、高pH、重金属、氧化等非生物胁迫的耐性,表明RcHSP17.8参与了上述非生物胁迫的响应。RcHSP1Z 8 is a high temperature （HT） induced expressing gene, by RT-PCR and Southern blot indicate that there are several copies of RcHSP1Z 8 in genome DNA of Rosa hybrida L. RcHSP1Z 8 is more strongly expressed in varieties resistant to HT, such as ＇ Schloss Mannieim＇ , ＇ Radio＇ , ＇ Las Vegas＇ , ＇ Caprice de Meiland＇ , ＇ Girl＇ , meanwhile weakly or not expressed in varieties sensitive to HT, such as ＇ Kordes＇ Perfecta＇ , ＇ Hiogi＇ , ＇ Pfalzer Gold＇. The results indicate that this gene is possibly relative to the tolerance in Rosa hybrida L. to HT. In order to study its possible function, RcHSP17.8 gene has been transferred into Escherichia coli BI21. Recombinant RcHSP17.8 was overexpressed in the recombinant E. coli cells which showed improved viability under different abiotic stress conditions, such as, high salt concentration, heavy metal and oxidative stress, as compared with control cultures. Those results would provide molecular identification principle to selecting varieties of Rosa hybrida L. with strong tolerance to HT.

关 键 词：月季 RcHSP17.8基因 诱导表达 非生物胁迫 

分 类 号：S685.12[农业科学—观赏园艺]