头皮针对脑缺血再灌注大鼠缺血区脑组织bcl-2、caspase-3蛋白表达以及血液流变的影响  

Effects of Electroacupuncture on Cerebral Bcl-2 and Caspase-3 Expression after Cerebral Ischemia Reperfusion in Rats

作　　者：陈锋[1] 严志康[1] 杨波[1] 

机构地区：[1]武汉大学人民医院针灸科,武汉430060

出　　处：《针刺研究》2009年第6期363-367,共5页Acupuncture Research

摘　　要：目的:探讨头皮针治疗缺血性脑卒中的作用机制。方法:48只SD大鼠随机分为假手术组、模型组、针刺组,每组16只。采用线栓法制备大鼠大脑中动脉缺血再灌注模型。针刺组取顶颞前斜线和顶颞后斜线,分别在造模后6、12、24h治疗,每次电针20min。运用免疫组化法观察再灌注24h后各组大鼠缺血区脑组织bcl-2、caspase-3蛋白的表达,全自动血流变仪测定血液流变各项指标表达情况。结果:脑缺血再灌注后大鼠海马回bcl-2和caspase-3蛋白表达明显升高(均P<0.05),全血黏度和红细胞聚集性明显升高(均P<0.05);针刺组和模型组比较,大鼠缺血区脑组织bcl-2表达水平增高,caspase-3表达水平降低(均P<0.05),针刺组大鼠全血黏度和红细胞聚集性明显低于模型组(均P<0.05)。结论:头皮针刺可以明显促进大鼠缺血区脑组织抑凋亡蛋白的表达,同时降低促凋亡蛋白的表达,改善血液流变性,这可能是针刺减轻脑缺血损伤的机制之一。Objective To investigate the underlying mechanism of electroacupuncture （EA） in anti-apoptosis effects in cerebral ischemia-reperfusion injury （CI/RI） rats. Methods Forty-eight SD rats were randomized into sham-operation （sham）, model and acupuncture groups with 16 cases in each. CI/RI model was established by occlusion of the middle cerebral artery for 2 h and reperfusion for 24 h. Neuronal apoptosis and expression levels of the cerebral bcl-2 and caspase-3 after administration of EA （MS 6, MS 7） were detected by immunohistochemistry. Whole blood viscosity, erythrocyte deformability and erythrocyte agreeability in rats after local CI/RI were assayed by using Automatic Blood Rheometer. Results In comparison with control group, bcl-2 and caspase-3 immunoactivity of the ischemic cerebral tissue, high, medium and low shear rates, aggregation index and rigidity index of the erythrocytes in model group increased significantly （P〈0.05）, while the erythrocyte deformity index reduced strikingly （P〈0.05）. Compared with model group, bcl-2 immunoactivity and erythrocyte deformity of acupuncture group increased remarkably （ P〈0.05）, and caspase-3 immunoactivity, high, medium and low shear rates of the whole blood viscosity, and erythrocyte aggregation index and rigidity index decreased considerably after acupuncture （P〈0.05）, suggesting an improvement of the erythrocyte deformability and agreeability after acupuncture. Conclusion Acupuncture can resist neuronal apoptosis during CI/RI, which may be closely associated with its effects in upregulating bcl-2 expression, downregulating caspase-3 expression. Moreover, acupuncture can significantly improve the erythrocyte deformability and reduce blood viscosity and erythrocyte agreeability.

关 键 词：脑缺血针刺 BCL-2 caspase-3血液流变 

分 类 号：R246.6[医药卫生—针灸推拿学]