电针不同穴位对脊髓损伤后尿潴留大鼠脊髓中脑源性神经营养因子及其酪氨酸受体激酶B表达的影响  

Effect of Electroacupuncture of Different Acupoints on the Excitability of Detrusor Muscle and the Expression of BDNF and TrkB in the Spinal Cord of Rats with Urinary Retention due to Spinal Cord Injury

作　　者：王俊华[1] 陈邦国[2] 尹晶[1] 王刚[1] 邹玮庚[1] 骆小娟[1] 

机构地区：[1]郧阳医学院附属太和医院康复医学科,湖北十堰442000 [2]湖北中医学院,武汉430061

出　　处：《针刺研究》2009年第6期387-392,共6页Acupuncture Research

基　　金：国家"973"计划项目"穴位效应规律的研究"的子课题(2005CB523308)

摘　　要：目的:研究电针不同穴位对脊髓损伤后尿潴留大鼠脊髓中脑源性神经营养因子(BDNF)及其酪氨酸受体激酶B(TrkB)表达水平和逼尿肌兴奋性的影响及作用机制,探讨不同穴位的相对特异性。方法:SD雌性大鼠随机分为5组:正常组、假手术组、模型组、电针关元穴治疗组(关元组)、电针水道穴对照组(水道组),每组20只。按照重物坠击方法建立脊髓损伤后尿潴留模型。关元组和水道组每天给予电针治疗,共治疗10d。通过肌条实验测定各组大鼠离体逼尿肌兴奋性,并采用免疫组织化学染色法检测损伤段脊髓中BDNF及其受体TrkB的表达水平。结果:模型组逼尿肌兴奋性比正常组和假手术组低,关元组和水道组均明显高于模型组(均P<0.05),且关元组优于水道组(P<0.05)。模型组脊髓中BDNF及其受体TrkB表达水平比正常组和假手术组高,关元组和水道组均明显高于其它组(均P<0.05),且关元组优于水道组(P<0.05)。结论:穴位电针治疗脊髓损伤后尿潴留的机制可能是通过促进脊髓中具有营养和修复神经功能的BDNF及TrkB表达的提高,使受损的脊髓神经通路得到修复,从而增强了逼尿肌排尿反射活动及其兴奋性。关元穴作用效应优于水道穴,说明不同穴位具有相对特异性。Objective To observe the effect of electroacupuncture （EA） of different acupoints on the expression of brain-derived neurotrophic factor （BDNF） and tropomyosine receptor kinase B （TrkB） in the spinal cord and the excitability of detrusor muscle of the uninary bladder in rats with urinary retention owing to spinal cord injury （SOl）. Methods A total of 100 female SD rats were randomly divided into normal, sham-operation （sham）, model, EA-Guanyuan （CV 4）, and EA-Shuidao （ST 28） groups, with 20 cases in each. SOl induced urinary retention model was established by using weight dropping method. EA （1 mA, 2 Hz/15 Hz） was applied to ＂Guanyuan＂ （CV 4） and ＂Shuidao＂ （ST 28） respectively for 20 min, once a day for 10 days. The excitability （tone, contraction frequency） of the detrusor muscle of the bladder was detected in vitro by using electrophysiological method, and the expression of BDNF and TrkB in spinal cord was determined by immunohistochemistry staining. Resuits In comparison with normal control and sham groups, the tension and the contraction frequency of detrusor muscle in model group lowered significantly （P〈0.05）, while compared with model group, both the tension and contraction frequency of detrusor muscle increased pronouncedly in EA-OV 4 and EA-ST 28 groups （ P〈0.05）, and the effect of EA-OV 4 was apparently superior to that of EA-ST 28 （P〈0.05）. In comparison with normal and sham groups, the BDNF and TrkB immunoreaction positive cells in the spinal cord were significantly more in model group （P〈0.05）. Compared with model group, those of EA-OV 4 and EA-ST 28 groups were obviously further increased （ P〈0.05）, and the effect of EA-OV 4 group was markedly superior to that of EA-ST 28 group（P〈0.05）. Conclusion EA of CV 4 and ST 28 can raise the excitability of the smooth muscle of the uninary bladder in rats with SOl-induced urinary retention, which may be related to its effects in upregulating the expression of BDNF and TrkB

关 键 词：脊髓损伤 尿潴留 电针 穴位特异性 逼尿肌兴奋性 脊髓脑源性神经营养因子 酪氨酸受体激酶B 

分 类 号：R245.97[医药卫生—针灸推拿学]