Dsg3刺激诱导天疱疮患者一级亲属T细胞活化的研究  

Study on Dsg3 induced activation of T cells in first degree relatives of patients with pemphigus vulgaris

作　　者：魏仲香[1,3] 耿龙[1] 胡婕[1] 孙峥嵘[4] 都姝妍[2] 姜奕[2] 

机构地区：[1]中国医科大学附属第一医院皮肤性病科,卫生部免疫皮肤病学重点实验室,沈阳110001 [2]中国医科大学附属第一医院中心实验室,沈阳110001 [3]山东聊城职业技术学院 [4]中国医科大学附属盛京医院病毒研究所

出　　处：《中华微生物学和免疫学杂志》2009年第12期1090-1093,共4页Chinese Journal of Microbiology and Immunology

基　　金：国家自然科学基金（30471567）;长江学者和创新团队发展计划（IRT0760）

摘　　要：目的研究在特异抗原桥粒芯糖蛋白3（desmoglein3，Dsg3）刺激下寻常型天疱疮（PV）患者一级亲属淋巴细胞Th1／Th2、Tc1／Tc2极化状态的变化，探讨PV发生机制。方法PV患者一级亲属外周血单个核细胞（PBMC）在Dsg3刺激下体外培养3d，流式细胞仪四色胞内染色分析PV患者一级亲属CD4^＋T细胞和CD8^＋T细胞中IFN-γ^＋和IL-4^＋细胞的百分率，观察PV患者一级亲属Th1／Th2、Tc1／Tc2比例的变化。结果PV抗体阳性一级亲属中的PBMC经Dsg3刺激培养者与未经Dsg3刺激者比较，Th2、Tc2百分率均显著升高（10．13％±3．72％VS7．28％±3．58％，20．01％±10．43％VS14．91％±8．06％，P〈0．05）；经Dsg3刺激培养后PV抗体阳性一级亲属组与正常对照组比较Th2、Tc2百分率均显著升高（10．13％±3．72％VS6．10％±2．82％，20．01％±10．43％VS9．58％±5．49％，P〈0．05）。结论特异性抗原Dsg3刺激下，自身反应性T细胞活化，Th1／Th2、Tc1／Tc2细胞分化失衡，可能在PV的启动阶段发挥重要作用。Objective To investigate The Th1/Th2 and Tc1/Tc2 polarization in the peripheral blood of first degree relatives of pemphigus vulgaris （PV） and healthy control individuals, and to approach the mechanism of the Dsg3-specific autoimmunity in PV. Methods The peripheral blood mononuclear cells （PBMC） from first degree relatives and healthy control was stimulated for 72 h with Dsg3 and without Dsg3. Th1/Th2, Tc1/Tc2 was assessed by four-color flow cytometry. Results The mean frequency of Dsg3-specific Th2 cells for PV antibody positive first degree relatives was 10. 13% ± 3.72%, compared with stimulation without antigen 7.28% ± 3.58%, the difference was significant （ P 〈 0.05 ）. The percentage Dsg3-specific Th2 was markedly higher in the PV antibody positive first degree relatives group than that in the control group（ 10. 13% ± 3.72% vs 6. 10% ± 2.82% , P 〈 0. 05 ） , Tc2 was markedly higher also （ 20.01% ± 10.43% vs 14.91% ±8.06%, 20.01% ±10.43% vs9.58% ±5.49%, P〈0.05）. Conclusion When Dsg3 stimulated PBMC were used to stimulate autologous T cells an increased amount of Th2 and Tc2 was observed. It is implied that the imbalance of Th1/Th2, Tc1/Tc2 might play an important role in the initiation of PV.

关 键 词：寻常型天疱疮 一级亲属 T淋巴细胞 DSG3 流式细胞术 

分 类 号：R686[医药卫生—骨科学]