龙眼成花逆转相关基因表达的cDNA-AFLP分析  

cDNA-AFLP Analysis of Gene Expression between Normal Flowering and Flowering Reversion Buds in Longan

作　　者：曾志[1,2] 王平[3] 梁文裕[2] 唐晰[2] 谢东丽[2] 郑少泉[4] 陈伟[1,2] 

机构地区：[1]福建农林大学作物遗传育种与综合利用教育部重点实验室,福州350002 [2]福建农林大学生命科学学院,福州350002 [3]福建农林大学园艺学院,福州350002 [4]福建省农业科学院果树研究所,福州350013

出　　处：《农业生物技术学报》2009年第6期1050-1055,共6页Journal of Agricultural Biotechnology

基　　金：国家自然科学基金项目(No.30571293);教育部博士点基金项目(No.200803890009);福建省自然科学基金项目(No.2007J0045)资助

摘　　要：以龙眼(Dimocarpus longan Lour.cv.Longyou)正常成花花芽和成花逆转花芽为材料,应用cDNA-AFLP技术,通过64对引物组合的cDNA-AFLP分析获得了2560条扩增片段,选择有明显差异的片段进行回收和测序,获得了14条核苷酸序列,其中有13个片段在GenBank数据库中发现同源序列,1个功能未知。通过核酸和氨基酸比对分析,112基因片段同源于银灰杨(Populus tremula×Populusalba)中编码Nek激酶家族(NIMA related protein kinases)基因(83%),331的核苷酸序列(80%)同源于与拟南芥(Arabidopsis thaliana)中编码内切-1,4-β-葡聚糖酶(EGases)的基因,313的核苷酸序列(78%)同源于与柑橘(Citrus sinensis)中的几丁质酶(chitinase CHI1)基因。提示这些基因片段在龙眼成花逆转过程中参与器官脱落、生殖生长和营养生长等的调控。In order to investigate the molecular mechanism of flowering reversion in Longan （Dimocarpus longan Lour, cv. Longyou）, differential expression profile of cDNA between the longan normal flowering and flowering reversion was analyzed by cDNA-AFLP. Sixty-four pairs of primer were used for the cDNA-AFLP amplification and totally 2 560 differentially expressed fragments were detected. Fragments with obvious difference were selected to re-amplification and purification, sequenced, and 14 sequences were obtained. According to the alignment of Blastn and Blastx, differentially expressed 112 gene fragments were 83% identical in nucleotides to Populus tremula x Populus alba NIMA-related protein kinase mRNA and 331 were 80% identical in nucleotides to Arabidopsis thaliana xyloglucan endo-1,4-beta-D-glucanase precursor mRNA and 313 were 78% identical in nucleotides to Citrus sinensis chitinase CHI1 （chi1） mRNA, indicating that these gene fragments relate to organ fall off, procreaction growth and nutrition growth during the flowering reversion in longan.

关 键 词：龙眼 成花逆转 基因差异表达 CDNA-AFLP 

分 类 号：S188[农业科学—农业基础科学]