芪术颗粒对大鼠肝纤维化形成过程中VEGF表达的影响  

Qizhu Granules downregulate the expression of vascular endothelial growth factor in carbon tetrachloride-induced liver fibrosis in rats

作　　者：时磊[1] 刘绍能[1] 李敏[1] 吕鑫霞[1] 阚杰[1] 马继征[1] 陈兰羽[1] 

机构地区：[1]中国中医科学院广安门医院,北京市100053

出　　处：《世界华人消化杂志》2009年第36期3675-3678,共4页World Chinese Journal of Digestology

基　　金：北京市自然基金资助项目;No.7072068~~

摘　　要：目的:动态观察芪术颗粒对肝纤维化模型大鼠肝组织血管内皮生长因子(VEGF)表达的影响.方法:应用CCl4复制大鼠肝纤维化模型,同时给予芪术颗粒灌胃预防,并设立空白对照组、模型对照组、鳖甲软肝片对照组,分别于造模1、2、3d及1、2、4、8、12wk各组取大鼠肝组织.实时荧光定量PCR方法检测VEGF mRNA的表达.结果:芪术颗粒组VEGF mRNA表达量在1、2、3d及1、2、12wk时较模型组低,有统计学差异(0.37±0.33vs0.48±0.26,0.24±0.20vs0.50±0.65,0.36±0.24vs0.45±0.29,0.25±0.14vs0.48±0.41,0.18±0.60vs0.30±0.30,0.40±0.01vs0.51±0.74,P<0.05或0.01).结论:芪术颗粒可通过下调实验大鼠肝脏VEGF mRNA的表达来抑制肝纤维化的产生.AIM： To observe the effects of Qizhu Granules on the expression of vascular endothelial growth factor （VEGF） in carbon tetrachloride （CCL4）-induced liver fibrosis in rats. METHODS： Rats were randomized into normal control group, model control group, Qizhu Granule treatment group and Compound Biejia Ruangan Tablet treatment group. After liver fibrosis was induced in rats by intraperitoneal injection of CCl4, Qizhu Granules and Compound Biejia Ruangan Tablets were given. Liver tissue samples were taken on days 1, 2 and 3 and at weeks 1, 2, 4, 8 and 12 for real-time fluorescence quantitative PCR detection of the expression of RESULTS： The expression levels of VEGF mRNA in Qizhu Granule treatment group on days 1, 2 and 3 and at weeks 1, 2 and 12 were significantly lower than those in the model control group （0.37±0.33 vs 0.48±0.26, 0.24±0.20 vs 0.50±0.65, 0.36±0.24 vs 0.45±0.29, 0.25±0.14 vs 0.48±0.41, 0.18±0.60 vs 0.30±0.30 and 0.40±0.01 vs 0.51±0.74; all P 〈 0.05 or 0.01）. CONCLUSION： Qizhu Granules can prevent the progression of liver angiogenesis and alleviate liver fibrosis possibly by downregulating the expression of VEGF mRNA in rats.

关 键 词：芪术颗粒 血管内皮生长因子 血管新生 

分 类 号：R285.5[医药卫生—中药学]