139例HIV感染者P_(24)抗原,HIV DNA,HIV RNA相关研究  

Study on the correlations of P 24 antigen,HIV DNA,HIV RNA in 139 HIV infected cases

作　　者：吴南屏[1,2] 刘克洲[1,2] 陈智 李继承[1,2] 

机构地区：[1]浙江医科大学传染病研究所 [2]德国吕贝克医科大学

出　　处：《中华微生物学和免疫学杂志》1998年第5期359-362,共4页Chinese Journal of Microbiology and Immunology

摘　　要：目的探讨ＨＩＶＤＮＡ，ＨＩＶＲＮＡ，Ｐ２４Ａｇ与宿主的相互关系及三者间的内在联系。方法收集１３９例抗ＨＩＶ阳性血标本，用固相免疫酶法测Ｐ２４Ａｇ，定量逆转录聚合酶链生物酶法测定ＨＩＶＲＮＡ和巢式聚合酶链法测ＨＩＶＤＮＡ。结果ＨＩＶＲＮＡ阳性数１０８／１３９，ＨＩＶＤＮＡ阳性数１０９／１３９，Ｐ２４Ａｇ阳性数为３４／１３９。进一步分析，同一标本ＨＩＶＤＮＡ，ＨＩＶＲＮＡ均阳性的占８０％，单一阳性为２０％。结论三种ＨＩＶ检测方法的敏感性、一致性与ＨＩＶ毒株变异、个体宿主免疫应答及抗病毒治疗密切相关。多引物多区域同时测ＨＩＶＤＮＡ，ＨＩＶＲＮＡ和抗原，并结合临床进行综合分析。Objective To study the relationship between the immune state of the host and P 24 Ag,HIV DNA,HIV RNA and their inner relations. Methods We collected 139 anti HIV seropositive blood samples to detect P 24 Ag, HIV DNA,HIV RNA by solidphase sandwichtype immunoassay(U.S.Abbott),nest PCR (Merke Germany) and RT PCR(U.S.RocheBra),respectively, Results The number of HIV DNA positive was 109/139 HIV RNA was 108/139,and P 24 Ag was 34/139,The further analysis:the rate of HIV DNA and HIV RNA both positive was 80%,20% samples had only single positive reaction. Conclusions We consider that the sensitivity and correspondence for detecting HIV are close related to the gene mutation of HIV strains,variety of individual immunoregulation and some treatment factors. So it is important to use more HIV primers to test HIV DNA,RNA and P 24 Ag simultaneously.The results of the test must be analyzed with clinical symptoms.It will help us to understand the mechanism of HIV infection and the result of treatment for AIDS.

关 键 词：DNA RNA 艾滋病病毒 P24抗原 

分 类 号：R373.9[医药卫生—病原生物学] R392.11[医药卫生—基础医学]