一种含噻吩基Ni(Ⅱ)配合物的合成及其与DNA的相互作用  

Synthesis and DNA Binding Property of a Thiophene Group Containing Nickel(Ⅱ) Complex

作　　者：蔡志彬[1] 周茂 

机构地区：[1]浙江工业大学精细化工研究所,杭州310014 [2]杭州赛利药物研究所,杭州310052

出　　处：《化学通报》2010年第1期75-78,共4页Chemistry

基　　金：浙江省分析测试科技计划项目(2007F70010)资助

摘　　要：以1,10-菲咯啉为原料,经氧化、缩合和络合反应,合成了一种含噻吩基的Ni(Ⅱ)配合物[Ni(phen)2TIP]2+,总收率为38.2%,并用MS和元素分析进行了结构表征。用电子吸收光谱和荧光光谱法研究了该配合物与小牛胸腺DNA的相互作用,其电子吸收光谱的最大吸收峰红移2nm,减色率为15.3%,同时配合物的荧光增强幅度为1.73。这些结果表明,配合物与DNA存在明显的插入结合作用。A nickel（ Ⅱ ） complex containing thiophene group [ Ni（phen）2 TIP ] ^2 ＋ was synthesized from 1,10- phenanthroline by oxidation, condensation and complexation with total yield of 38.2% . Its structure was characterized by MS and elemental analysis. The binding of the complex to calf thymus DNA was investigated with electronic absorption and luminescenee spectra, The maximal electronic absorption peak of the complex was red- shifted （△λ = 2 nm） and the hypoehromism was 15.3%. Meanwhile, the fluorescence intensity increased 1.73 times. These results indieated that the complex was bonded to calf thymus DNA through an interealative mode.

关 键 词：Ni(Ⅱ)配合物 DNA 合成 插入作用 

分 类 号：O621.13[理学—有机化学]