利用罗非鱼头提取硫酸软骨素的工艺探讨  

Study on the extraction technology of chondroitin sulfate form tilapia skull

作　　者：李川[1] 段振华[1] 

机构地区：[1]海南大学食品学院,海口570228

出　　处：《食品科技》2010年第1期235-238,共4页Food Science and Technology

基　　金：国家科技支撑计划项目(2007BAD76B06)

摘　　要：以罗非鱼头为原料,初步探讨了硫酸软骨素的提取工艺,采用稀碱提取,酶解,三氯乙酸沉淀蛋白,乙醇沉淀的方法,考察了碱提取过程中碱浓度、料液比、温度、时间对提取率的影响,在单因素实验的基础上,利用正交实验进行了优化。得出了在碱浓度1%、料液比1∶5、提取温度50℃、提取时间7h,最大的得率为3.757%。The articles introduced a method of preparation chondroitin sulfate form tilapia, skull the effects of alkali condition, ratio of solid to liquid, temperature and time on the extraction rate during the alkali extraction was studied by using alkali extraction, enzyme hydrolysis, the precipitation of proein by trichioroacetic acid and of chondroitin sulfate by ethanol, on the basis of single-factor tests these parameter of extraction process was optimized by orthogonal test. The results showed that the alkali concentration was 1%, the ratio of powdered shull of tilapia to alkali was 1：5, tempeture was 50 ℃ and the extraction of dilute alkali was 7 h, the extraction percent of chondroitin sulfate conld be improved up to 3.757%.

关 键 词：罗非鱼 硫酸软骨素 碱提取 优化 

分 类 号：R284.2[医药卫生—中药学]