Isolation and Characterization of a Bensulfuron-Methyl-Degrading Strain L1 of Bacillus  

作　　者：LIN Xiao-Yan WANG Yi WANG Hai-Long T. CHIRKO DING Hai-Tao ZHAO Yu-Hua 

机构地区：[1]Department of Microbiology, College of Life Sciences, Zhejiang University, Hangzhou 310058 (China) [2]China National Rice Research Institute, Hangzhou 310006 (China) [3]Ensis, Rotorua Private Bag 3020 (New Zealand) [4]College of Environmental Sciences and Technology, Zhejiang Forestry University, Lin'an 311300 (China)

出　　处：《Pedosphere》2010年第1期111-119,共9页土壤圈（英文版）

基　　金：Project supported by the National High Technology Research and Development Program of China (863 Program)(No. 2007AA06Z329);the Science and Technology Program of Zhejiang Province (Nos. 2007C23036 and 2008C13014-3);the International Cooperation Program in Science and Technology of Zhejiang Province (No. 2008C14038)

摘　　要：The objectives of this study were to isolate a bensulfuron-methyl （BSM）-degrading strain of Bacillus spp. and to evaluate its effectiveness in remediation of a BSM-contaminated soil. A BSM-degrading bacterium, strain L1, was successfully isolated in this study. Strain L1 was identified as Bacillus megaterium based on its morphological, physiological, and biochemical properties, G＋C content, phylogenetie similarity of 16S rDNA, and fatty acid composition. Two experiments were used to examine BSM degradation by strain L1. When BSM was used as a sole carbon source in a mineral salt medium, the average degradation rate of BSM by strain L1 was 12.8%, which suggested that the strain was able to utilize BSM as a sole carbon and energy source. Supplement of yeast extract （200 mg L^-1） significantly （P ≤ 0.01） accelerated the degradation of BSM by strain L1. Ahnost complete degradation （97.7%） of BSM could be achieved in 84 h with addition of yeast extract. In addition, in a sterile soil with 50 mg L^-1 BSM, BSM degradation rate by strain L1 was 94.3% in 42 d, indicating the potential of using microbes for the remediation of BSM-contaminated soils in fields.The objectives of this study were to isolate a bensulfuron-methyl (BSM)-degrading strain of Bacillus spp. and to eval-uate its effectiveness in remediation of a BSM-contaminated soil. A BSM-degrading bacterium, strain L1, was successfully isolated in this study. Strain L1 was identified as Bacillus megaterium based on its morphological, physiological, and biochemical properties, G+C content, phylogenetic similarity of 16S rDNA, and fatty acid composition. Two experiments were used to examine BSM degradation by strain L1. When BSM was used as a sole carbon source in a mineral salt medium, the average degradation rate of BSM by strain L1 was 12.8%, which suggested that the strain was able to utilize BSM as a sole carbon and energy source. Supplement of yeast extract (200 mg L-1 ) significantly (P ≤ 0.01) accelerated the degradation of BSM by strain L1. Almost complete degradation (97.7%) of BSM could be achieved in 84 h with addition of yeast extract. In addition, in a sterile soil with 50 mg L-1 BSM, BSM degradation rate by strain L1 was 94.3% in 42 d, indicating the potential of using microbes for the remediation of BSM-contaminated soils in fields.

关 键 词：BIODEGRADATION carbon source phylogenetic analysis 16S rDNA 

分 类 号：TS221[轻工技术与工程—粮食、油脂及植物蛋白工程] Q939.124[轻工技术与工程—食品科学与工程]