HBV截短core基因融合preS1基因在BCG中的表达及其免疫应答  

Immune response in BALB/c mice immunized with BCG expressing HBV truncated C gene and preS1 gene

作　　者：尹文[1] 吕欣[2] 雷迎峰[2] 杨敬[2] 孙梦宁[2] 马玉[2] 兰海云[1] 汪爱勤[1] 

机构地区：[1]第四军医大学基础部中心实验室,陕西西安710032 [2]第四军医大学基础部微生物学与病原生物学教研室,陕西西安710032

出　　处：《细胞与分子免疫学杂志》2010年第1期28-30,共3页Chinese Journal of Cellular and Molecular Immunology

基　　金：国家自然科学基金资助项目(30671878;30670100)

摘　　要：目的:探讨HBV相关抗原在卡介苗(BCG)中的有效表达及其刺激产生的体液和细胞免疫应答效果。方法:将带有HBV截短C基因和preS1基因片段的穿梭载体转化BCG菌株,筛选重组菌,SDS-PAGE和Western blot实验分析其抗原表达特性;分别以生理盐水、BCG、BCG-pDE22、BCG-pDE22-CS1皮下注射BALB/c小鼠,进行连续抗体测定和细胞毒性T细胞杀伤实验。结果:与对照组相比,重组BCG可表达Mr为24000的新生蛋白,与CS1融合蛋白大小相符,Westernblot实验分析显示出良好的抗原结合特性;带有HBVCS1抗原基因的重组BCG免疫组抗体滴度显著升高,特异性杀伤能力更强。结论:BCG可以作为HBV相关抗原的活载体,为研制HBV新型疫苗提供了实验依据。AIM：To study expressing of HBV truncated core and preS1 protein in BCG and to explore the effect of humoral and cellular immune response stimulated by the recombinant BCG.METHODS：A shuttle vector was constructed and was transformed into BCG which including truncated Core gene and preS1 gene of HBV.The recombinant BCGs were analyzed with SDS-PAGE and Western blot.The three groups of BALB/c mice were immunized with saline,BCG,BCG-pDE22 and BCG-pDE22-CS1 respectively.Then the antibody titer and CTL effects were evaluated.RESULTS：Compared with the control group,the recombinant BCG could express a new protein band of 24 kD which was consistent with the size of CS1 fused protein which displayed a good antigen-binding property by Western blot analysis.The antibody titer significantly increased and CTL effect apparently enhanced in the recombinant BCG immunized group.CONCLUSION：BCG could be as live vector which carrying HBV related genes,which developing a novel vaccine against HBV.

关 键 词：乙型肝炎病毒 细胞毒性T细胞 BCG 抗体 

分 类 号：R392.11[医药卫生—免疫学]