转hpa1_(Xoo)基因棉花对棉铃虫全基因组转录谱的影响  

Genome-wide Microarray Analysis of Helicoverpa armigera Larva Fed on Transgenic hpa1_(Xoo) Cotton Leaves

作　　者：缪卫国[1] 武淑文[1] 宋从凤[1] 汪瑜[1] 龚晓崇[1] 张亮 王金生[1] 

机构地区：[1]南京农业大学植物保护学院/农业部作物病虫害监测与防控重点开放实验室,南京210095 [2]博奥北京国家生物芯片公司,北京102206

出　　处：《中国农业科学》2010年第2期313-321,共9页Scientia Agricultura Sinica

基　　金：国家自然科学基金项目(30230240);国家重点基础研究发展规划资助项目(G2006CB101902);国家"十一五"科技支撑项目(2004BA901A36);国家高技术研究和发展计划(863-2006AA02Z180)

摘　　要：【目的】明确转hpa1Xoo棉花抗虫性及其作用机制。【方法】采用生测法和芯片技术分析了转hpa1Xoo棉T-34的抗虫性和harpinXoo表达对棉铃虫全基因组转录谱的影响。【结果】饲喂T-34棉叶的棉铃虫发育明显迟缓,棉铃虫死亡率为90.95%,而饲喂其出发品种(Z35)棉叶的棉铃虫死亡率为12.20%。利用和棉铃虫同属鳞翅目的家蚕的23KOligo探针,以分别饲喂T-34和Z35叶片的棉铃虫RNA为模板,制作了棉铃虫Oligo表达谱芯片,获得了24280个EST数据,从中检测到了2927个基因。在T-34和Z35上饲喂的棉铃虫有872个差异表达基因,其中上调基因328个(ratio>2.0),下调基因544个(ratio<0.5)。872个差异表达基因主要涉及13个生物功能、96个代谢通路。【结论】转hpa1Xoo棉花对棉铃虫的抗性涉及棉铃虫蛋白酶分解和合成、ATP合成等多个生物代谢途径的改变。[Objective ] The objective of this study was to confirm the resistance of transgenic hpalxoo cotton T-34 to cotton bollworm （Helicoverpa armigera） and its resistance mechanism. [Method] Bioassay and 23K Oligo silkworm microarray were used to analyze the insect resistance of T-34 and globe transcriptome profile of H. armigera kept by transgenic hpalxoo cotton leaves comparing to untransformed cotton Z35. [Result] The fresh leaves of T-34 and Z35 were utilized to keep H. arrnigera larva, and only T-34 made H. armigera larval development become slow rather than Z35. Microarray data indicated that 872 genes in H. armigera were totally deregulated by the comparison of T-34 vs Z35. Of 872 genes, 328 genes were showed to be upregulated （ratio〉2.0）, and 544 genes were indicated to be downregulated （ratio〈0.5）. It was suggested that all differentially expressed genes might be classified and related to 13 biological functions, and involved in 96 biological pathways. [Conclusion] harpinxoo encoded by hpa1xoo confers the resistance of transgenic hpa1xoo cotton to H. arrnigera, and influences the multiple metabolism pathways in H. armigera larva.

关 键 词：转hpa1xoo棉花 harpinloo HELICOVERPA ARMIGERA 家蚕01igo芯片 差异表达基因 

分 类 号：S435.622[农业科学—农业昆虫与害虫防治]