一株类猪圆环病毒2型因子P1的全基因组序列测定与分析  

Sequencing and Analysis of Complete Genome of a Porcine Circovirus Type 2-like Agent P1

作　　者：温立斌[1] 何孔旺[1] 杨汉春[2] 

机构地区：[1]江苏省农业科学院兽医研究所,南京210014 [2]中国农业大学动物医学院,北京100193

出　　处：《中国农业科学》2010年第2期411-416,共6页Scientia Agricultura Sinica

基　　金：国家自然科学基金资助项目(30972184);国家"973"计划前期研究专项资助课题(2007CB116308)

摘　　要：【目的】确定猪体内存在与猪圆环病毒2型核苷酸序列高度同源的因子。【方法】首先利用PCR方法对来自临床表现为猪断奶后多系统衰竭综合征的猪血清所抽提的DNA进行扩增,根据获得的序列再设计引物进行反向PCR,拼接得到类猪圆环病毒2型因子P1的全基因组序列,最后根据P1全基因组序列设计引物扩增其全基因组加以验证。【结果】首次完成了类猪圆环病毒2型因子P1的全基因组序列测定。测序结果表明P1因子为环状DNA基因组,全长648个核苷酸,包括3个开放阅读框。除5′端16个核苷酸外,P1因子与国内猪圆环病毒2型BF毒株的核苷酸同源率为98.42%。系统进化分析表明P1与猪圆环病毒有很近的亲缘关系。【结论】猪体内存在类猪圆环病毒2型因子P1。[ Objective ] The aim of the experiment is to discover an agent containing high homologous sequences of porcine circovirus type 2 in swine. [Method] PCR was developed for detection of DNA extracted from porcine sera collected from pigs with clinical signs of postweaning multisystemic wasting syndrome, then a reverse PCR was carded out based on the acquired sequences in this study, the sequences of PCR fragments were assembled into consecutive sequence and then finally verified by amplifing the agent complete genome. [Result] Complete nucleotide sequence of genome of porcine circovirus type 2-like agent P1 was firstly reported in this paper. P1 contained circular genomic DNAs of 648 nucleotides and three open reading frames. Except 16 nucleotides in the 5＇ end of the genome, the genome of P 1 showed 98.42% of nucleotide homology compared with porcine circovirus type 2 BF isolate. Phylogenetic analysis revealed that P1 was closely related to the porcine circovirus. [Conclusion] Results suggested that there existed porcine circovirus type 2-like agent P1 in swine.

关 键 词：猪圆环病毒2型 P1 全基因组 序列测定与分析 

分 类 号：S852.65[农业科学—基础兽医学]