薄层色谱扫描法检测血清中对乙酰氨基酚  

Determination of acetaminophen in serum with thin layer chromatography scanning method

作　　者：李贵明[1,2] 安健康[1,2] 贺娟[1,2] 员克明[1,2] 

机构地区：[1]山西医科大学法医系 [2]山西省心血管病研究所

出　　处：《中国医院药学杂志》1998年第10期452-453,共2页Chinese Journal of Hospital Pharmacy

摘　　要：目的：本研究拟采用薄层色谱扫描法建立快速测定患者血清中对乙酰氨基酚浓度的方法，为指导临床合理用药和中毒急救提供依据。方法：实验取１００μｌ血清，用蒸馏水稀释１０倍，再取１００μｌ稀释血清用乙醚提取，４０℃水浴挥干，残渣用乙醇溶解点样，展开后以λ测２４５ｎｍ，λ参３１０ｎｍ薄层扫描测定。结果：本法板间测定误差小，回收率高，线性范围宽，能准确测定患者血清浓度。结论：这一方法与文献所记载的ＨＰＬＣ和ＧＣ相比，具有简便快速、费用低和不需要做衍生化的特点。Objective:The purpose of the study is to establish a method for monitoring serum concentration of acetaminophen with thin layer chromatography scanning method(TLCS),and therefore to guide reasonable administration and treatment for cases who are poisoned with acetaminophen;Methods:This experiment used 100 μl serum of patients with acetaminophen poisoning and diluted to 1 ml,finally 100 μl diluted serum were taken to determinated the serum level of acetaminophen.The procedure was that the first step was the extraction with ether and evaporation in 40℃ water bath, the second was to scan the spots at the wave length of λ245 nm and 310 nm on the thin layer plate after spoting and developing.Results:The determination variations among plates were small with higher recoveries, wider linear range TLCS, compared with HPLC and GC, was simple, rapid, and did not need to derivate acetaminophen.It is suitable for emergency analysis and drug monitoring for acctaminophen.

关 键 词：对乙酰氨基酚 薄层色谱法 血药浓度 

分 类 号：R971.1[医药卫生—药品] R969.1[医药卫生—药学]