两个品种转基因抗虫棉光合生理的CO_2响应  

Responses of photosynthetic physiological characteristics of two transgenic cotton (Gossypium hirsutum L.) varieties to CO_2 concentration

作　　者：孙彩霞[1] 郝健均[1] 王杰[1] 缪璐[1] 陈振华[2] 齐华[3] 

机构地区：[1]东北大学理学院,沈阳110004 [2]中国科学院沈阳应用生态研究所,沈阳110016 [3]沈阳农业大学农学院,沈阳110161

出　　处：《生态学报》2010年第2期504-510,共7页Acta Ecologica Sinica

基　　金：辽宁省博士启动基金资助项目(20041008);辽宁省科技攻关资助项目(2004201003);国家自然科学基金资助项目(40101016)

摘　　要：栽培环境条件的改变会对转基因作物产生深远影响。以2种不同转基因棉花及其亲本对照为材料,研究了盆栽种植条件下不同棉花品种在蕾期和吐絮期光合生理特性CO2响应特征。结果表明,与各自的常规棉对照比较,两种转基因抗虫棉单叶净光合速率CO2响应的特征参数表观初始羧化效率(CE)、表观暗呼吸速率(Rd)和最大净光合速率(Pmax,c)虽有一定程度的变化,但其差异均未达到显著水平。在高CO2浓度范围内(>700μmol.mol-1),转基因抗虫棉单叶净光合速率和水分利用率(WUE)的CO2响应曲线特征发生变化,且与品种及生育时期有关。两种转基因抗虫棉在不同生育时期的气孔导度(Gs)对CO2浓度的响应特征与其常规棉对照相似,短期CO2浓度增高对转基因抗虫棉的气孔导度没有显著性影响。Changes in environmental conditions could have an impact on genetically modified crops.The responses of photosynthetic physiological characteristics to CO2 in mature cotton leaves of two transgenic cotton varieties（transgenic Bt cotton Z30 and transgenic CpTI-Bt cotton SGK321）and their non-transgenic parental counterparts（Z16 and SY321）were investigated under different CO2 concentration conditions（0,50,75,100,200,300,400,600,800μmol·mol-1 and 1000μmol·mol-1）at squaring and boll-opening stages.The results showed that the parameters of apparent initial carboxylation efficiency,the maximum net photosynthetic rate and apparent dark respiration rate that calculated by model fitting in two transgenic cotton varieties were not significantly altered compared with those in parental counterparts.The obvious changes of responses of net photosynthetic rate in single leaf and water use efficiency to CO2 in Bt and CpTI-Bt transgenic cotton varieties were related to transgenic cotton genotypes and developmental stages at high CO2 concentration（above 700μmol·mol-1）.At different developmental stages,no obvious differences in responses of stomatal conductance to CO2 between transgenic cotton varieties and their parental counterparts were observed,suggesting that higher CO2 concentration had no significant short-term effects on stomatal conductance of transgenic cottons.

关 键 词：抗虫棉 光合速率 气孔导度 水分利用率 CO2浓度 

分 类 号：S562[农业科学—作物学] S188