高压蒸气灭菌同种异体骨移植的实验研究  

Experiment study on the transplantation with sterile bone allograft after autoclaving

作　　者：刘龙平[1] 胡小令[1] 李拾林[1] 

机构地区：[1]江西医学院解剖学教研室

出　　处：《中国临床解剖学杂志》1998年第4期341-345,共5页Chinese Journal of Clinical Anatomy

摘　　要：目的：观察高压蒸气灭菌同种异体骨在骨移植中的作用，探索简便、经济的骨移植方法。方法：用家用压力锅，对同种异体鼠骨高压蒸气灭菌３０ｍｉｎ后，将其植入２５只成年大白鼠双侧胫骨（新生鼠骨植入右侧１２例，成年鼠骨植入左侧２５例）前上部一个长１０ｍｍ，深２ｍｍ的缺损中，于术后３、６、９、１２周取材，制片，在普通、荧光光镜和透射电镜下进行观察。结果：发现经高压蒸气灭菌后的同种异体骨有较好的生物相容性，可以引导骨生长。结论：高压蒸气灭菌处理同种异体骨，是一种简便、经济的骨移植方法。Objective:To study the effect of autoclaving on allograft bone transplantation and to find a simple and economic methods for bone transplantation.Methods:Thirty-seven allografts bone were transplanted into the defect,which is 10 mm long and 3 mm deep,on both sides of the tibia in 25 rats,after being sterilized in domestic autoclave for 30 minutes.The rats were killed 3 weeks,6 weeks,9 weeks and 12 weeks after the operation and the specimens were observed under optic,fluorescent and transmission electron microscope.Results:The sterile allograft bone has good biocompatibility and is able to conduct the progressive growth of the bone.Conclusions:Transplantation with sterile allograft bone after autoclaving is one of the simple,economic methods,it is easy to spread in the rural and local area.

关 键 词：高压蒸气灭菌 同种异体移植 骨骼移植 

分 类 号：R687.34[医药卫生—骨科学] R187.5[医药卫生—外科学]