高聚物型色谱柱分离乙酰半胱氨酸及其有关物质  

Detection of relevant substances from acetylcysteine by HPLC

作　　者：方硕[1] 魏荣卿[1] 刘晓宁[1] 尹亚萍[1] 

机构地区：[1]南京工业大学生物与制药工程学院色谱中心,南京210009

出　　处：《分析试验室》2010年第1期25-28,共4页Chinese Journal of Analysis Laboratory

基　　金："863"项目(2007AA02Z200);"十一五"国家科技支撑计划(2006BAK03A06)项目资助

摘　　要：考察高聚物型色谱柱（聚苯乙烯-二乙烯基苯（PS-DVB）麦科菲反相高效液相色谱柱,MKF-RP-MH）分离乙酰半胱氨酸以及有关物质的适用性。采用MKF-RP-MH色谱柱（4.6×250 mm,5μm）,V（0.02 mol/L的磷酸盐缓冲液（pH 3.0））∶V（乙腈）=9∶1为流动相,流速为1 mL/min,检测波长210 nm,柱温为25℃。乙酰半胱氨酸峰与有关物质峰的分离度均大于1.5;乙酰半胱氨酸质量浓度在11～111 mg/L的范围内,具有良好的线性关系,回归方程为A=18.195ρ＋0.5229,r=0.9995（n=8）;乙酰半胱氨酸的定量限为11 mg/L;最低检测限为0.035μg;进样精密度RSD=0.24%（n=6）。To review the applicability of determination of relevant substances in acetylcysteine by HPLC with polystyrene-divinylbenzene（PS-DVB） resin（MKF-RP-MH）.The MKF-RP-MH（250 mm×4.6 mm,5 μm） column was used.The mobile phase was 0.02 mol/L phosphate buffer（pH 3.0）： acetonitrile（9∶1,V/V）,the flow rate was 1.0 mL/min,the detection wavelength was set at 210 nm,and column temperature was set at 25 ℃.The resolution between the acetylcysteine peak and relevant substances were not less than 1.5.The calibration curve was in good linearity within the range of 11 - 111 mg/L with the regression equation of A = 185.94p ＋ 0.0548, r = 0.9996 （ n = 8）. The quantitation limit was 11 rag/L, and the detection limit was 0.035 p.g. The prevision of the method was 0.24% （n =6）.

关 键 词：高聚物型色谱柱 聚苯乙烯-二乙烯基苯 MKF-RP-MH 乙酰半胱氨酸 有关物质 

分 类 号：O658.1[理学—分析化学]