耐甲氧西林金黄色葡萄球菌SCCmec基因分型研究与耐药基因检测  

Genotyping of SCCmec and Detection of Antibiotic-resistant Genes in Meticillin-resistant Staphylococcus aureus

作　　者：茆海丰[1,2] 邵世和[1] 杨晋[2] 赵勇[2] 营丽娟[2] 

机构地区：[1]江苏大学基础医学与医学技术学院,江苏镇江212013 [2]连云港市第一人民医院检验科,江苏连云港222002

出　　处：《中华医院感染学杂志》2010年第1期12-15,共4页Chinese Journal of Nosocomiology

摘　　要：目的了解耐甲氧西林金黄色葡萄球菌(MRSA)的葡萄球菌盒染色体(SCCmec)基因型分布特点和耐药基因的携带状况。方法采用多重聚合酶链反应(PCR)对医院分离的125株MRSA进行SCCmec基因型分型,普通PCR对其中的50株MRSA进行6种耐药基因扩增。结果检测的125株MRSA中124株SCCmec基因型为Ⅲ型,1株不能分型,6种耐药基因的阳性率分别为aac(6′)/aph(2″)98%、aph(3′)Ⅲ基因46%t、etM基因72%、erm基因86%,未扩增出TEM和ant(4′、4″)基因。结论引起感染的MRSA的基因型基本为SCCmecⅢ型,SC-CmecⅢ型的MRSA对氨基糖苷类、红霉素类、四环素类耐药基因有较高的携带率,多重PCR法可以有效的用于大批量临床分离的MRSA的SCCmec分型研究。OBJECTIVE To investigate the staphylococcal cassette chromosome mec （SCCmec） genotype characteristics and antibiotic-resistant genes in meticillin-resistant Staphylococcus aureus （MRSA） isolated from Lianyungang. METHODS The SCCmec of clinically isolated MRSA strains were genotyped with a novel multiplex PCR strategy reported by Zhangetal. Ant/biotic-resistant genes of aac（6′）/aph （2″）, aph（3′） Ⅲ, terM, erm, TEM, and ant（4′,4″） were analyzed by traditional PCR. RESULTS The isolates were almost SCCmec Ⅲ positve, only one isolate couldn＇t be typed. The positive rates of aac（6′）/aph（2″）,aph（3′） Ⅲ ,terM, and erm were 98%,46%, 72% and 86%, respectively. TEM and ant（4′,4″） tested were all negative. CONCLUSIONS Almost all genotypes of MRSA prevailing in Lianyungang carry the SCCmec Ⅲ gene. There are high positive percentages of antibioticresistant genes of aac（6′）/aph（2″）, aph（3′）//], tetM and erm in the isolates. The novel multiplex PCR strategy recommended by Zhang et al can be applied into genotyping study of MRSA SCCmec effectively.

关 键 词：耐甲氧西林金黄色葡萄球菌 葡萄球菌盒染色体 耐药基因 

分 类 号：R378.11[医药卫生—病原生物学]