肺炎克雷伯菌对季铵盐消毒剂及复方新诺明耐药相关基因检测  

Detection of Quaternary Ammonium Compounds-resistant Gene and Sulfamethoxazole/trimethoprim-resistant Gene in Klebsiella pneumoniae

作　　者：储秋菊[1] 杨海燕[1] 夏守慧[1] 杨伟平[1] 盛以泉[1] 葛丽卫[1] 糜祖煌[2] 黄支密[1] 

机构地区：[1]解放军第98医院检验科,浙江湖州313000 [2]无锡市克隆遗传技术研究所,江苏无锡214026

出　　处：《中华医院感染学杂志》2010年第2期157-160,共4页Chinese Journal of Nosocomiology

摘　　要：目的了解临床分离的肺炎克雷伯菌（KPN）季铵盐消毒剂及复方新诺明耐药相关基因存在状况。方法在2005年9月-2006年4月从住院患者中分离出25株肺炎克雷伯菌，采用PCR及序列分析的方法分析5种基因qacE△1-sull、dfrA1、dfrA5、dfrA12和dfrA17。结果25株肺炎克雷伯菌中，qacE△1-sull、dfrA1、dfrA5、dfrA12和dfrA17基因阳性株数分别为21（84．0％）、0、6（24．0％）、6（24．0％）和0；qacE△1-sull阳性基因测序比对与已在美国核苷酸序列数据库（GenBank）中登录的qacE△1-sull基因序列完全同源。结论临床分离的肺炎克雷伯菌中qacE△1-sull基因阳性率很高，对复方新诺明耐药与菌株携带dfrA5、dfrA12和qacE△1-sull基因有关。OBJECTIVE To investigate the presence of the quaternary ammonium compounds-resistant gene and sulfamethoxazole/trimethoprim-resistant genes in Klebsiella pneumoniae isolated from the PLA 98th Hospital, Huzhou,Zhejiang province. METHODS Twenty-five strains of K. pneumoniae were isolated from the inpatients from Sep 2005 to Apr 2006. Five kinds of genes of qacE△l-sull ,dfrA1 ,dfrA5 ,dfrA12 and dfrA17 were analyzed by PCR and verified by DNA sequencing. RESULTS In 25 strains of K. pneurnoniae,the positive rate of genes of qacE△1-sull, dfrA5 and dfrA12 were 84.0% ,24.0% ,and 24.0% ,respectively. Both genes of dfrA1 and dfrA17 were tested negative. The total positive rate of 5 kinds of genes was 92.0% （23/25）. qacEA1-sull Positive gene sequencing showed highly homology with the sequence of GenBank （GenBank Accession Number： U12338）. CONCLUSIONS There is very high positive percentage of qacEA1-sull gene in K. pneumoniae isolated from the inpatients. There is a close relation of the sulfamethoxazole/trimethoprim resistance in K. pneumoniae to qacE△1- sull,dfrA5 and dfrA12 existing.

关 键 词：肺炎克雷伯菌 季铵盐复合物 复方新诺明 耐药 基因 

分 类 号：R378.996[医药卫生—病原生物学]